PRODUCTION OF HETEROKARYONS BY THE FUSION OF MESOPHYLL PROTOPLASTS OF PORTERESIA-COARCTATA AND CELL SUSPENSION-DERIVED PROTOPLASTS OF ORYZA-SATIVA - A NEW APPROACH TO SOMATIC HYBRIDIZATION IN RICE

被引：19

作者：

FINCH, RP

SLAMET, IH

COCKING, EC

机构：

[1] Plant Genetic Manipulation Group., Department of Botany, University of Nottingham, Nottingham

来源：

JOURNAL OF PLANT PHYSIOLOGY | 1990年 / 136卷 / 05期

关键词：

2[N-Morpholino]ethanesulfonic Acid; AC; alternating current; DC; direct current; FDA; flow cytometry; fluorescein diacetate; fresh weight; FW; MES; Oryza sativa; Porteresia coarctata; protoplast fusion; salt tolerance; ultra violet light; UV;

D O I：

10.1016/S0176-1617(11)80219-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A procedure is described for the electrofusion of protoplasts isolated from rice (Oryza sativa L.) cell suspension cultures with protoplasts isolated from leaves of Porteresia coarctata, a salt tolerant wild relative of cultivated rice. The frequency of heterokaryon formation was assessed by flow cytometry. When heterokaryons were cultured without selection they regenerated a cell wall and divided to form microcolonies. This new procedure for the somatic hybridization of these two sexually incompatible species is providing a novel approach to the production of salt tolerant cultivated rice. © 1990, Gustav Fischer Verlag, Stuttgart. All rights reserved.

引用

页码：592 / 598

页数：7

共 13 条

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