SERIAL PROPAGATION OF PORCINE ENTERIC CALICIVIRUS IN A CONTINUOUS CELL-LINE EFFECT OF MEDIUM SUPPLEMENTATION WITH INTESTINAL CONTENTS OR ENZYMES

被引：47

作者：

PARWANI, AV ^{[1
]}

FLYNN, WT ^{[1
]}

GADFIELD, KL ^{[1
]}

SAIF, LJ ^{[1
]}

机构：

[1] OHIO STATE UNIV,OHIO AGR RES & DEV CTR,FOOD ANIM HLTH RES PROGRAM,WOOSTER,OH 44691

来源：

ARCHIVES OF VIROLOGY | 1991年 / 120卷 / 1-2期

关键词：

D O I：

10.1007/BF01310954

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A porcine enteric calicivirus (PEC) was adapted to serial propagation in a continuous porcine kidney cell line (LLC-PK) by inclusion of an intestinal contents preparation (ICP), derived from uninfected gnotobiotic pigs, in the maintenance medium. Infected cells were detected by immunofluorescent staining of cell monolayers. Attempts to propagate PEC in the presence of various intestinal enzymes of porcine origin, bacterial protease, bovine ICP, avian ICP or without medium supplementation were unsuccessful.

引用

页码：115 / 122

页数：8

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