PCR-BASED IMMUNOGENOTYPING AT THE IG HEAVY-CHAIN CDR3 LOCUS - IMPROVEMENTS IN RESOLUTION

被引：22

作者：

POLLARD, P

OWEN, G

WORWOOD, M

机构：

[1] UNIV HOSP WALES,DEPT CHILD HLTH,CARDIFF CF4 4XW,S GLAM,WALES

[2] UNIV WALES COLL MED,CARDIFF CF4 4XN,S GLAM,WALES

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1993年 / 84卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1111/j.1365-2141.1993.tb03043.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

An improved electrophoretic separation of products after PCR employing consensus primers to amplify the CDR3 of the immunoglobulin heavy chain variable region allows visualization of a ladder of fragments in polyclonal samples of peripheral blood as well as a single band where there is clonal proliferation.

引用

页码：169 / 171

页数：3

共 8 条

[1] DEVELOPMENT OF A HIGHLY SENSITIVE ASSAY, BASED ON THE POLYMERASE CHAIN-REACTION, FOR RARE LYMPHOCYTE-B CLONES IN A POLYCLONAL POPULATION
BRISCO, MJ
TAN, LW
ORSBORN, AM
MORLEY, AA
[J]. BRITISH JOURNAL OF HAEMATOLOGY, 1990, 75 (02) : 163 - 167
[2] DETECTION OF IMMUNOGLOBULIN GENE REARRANGEMENT IN B-LYMPHOID MALIGNANCIES BY POLYMERASE CHAIN-REACTION GENE AMPLIFICATION
DEANE, M
NORTON, JD
[J]. BRITISH JOURNAL OF HAEMATOLOGY, 1990, 74 (03) : 251 - 256
[3] GRIESSER H, 1989, BLOOD, V73, P1402
[4] A SIMPLE SALTING OUT PROCEDURE FOR EXTRACTING DNA FROM HUMAN NUCLEATED CELLS
MILLER, SA
DYKES, DD
POLESKY, HF
[J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (03) : 1215 - 1215
[5] PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE
SAIKI, RK
GELFAND, DH
STOFFEL, S
SCHARF, SJ
HIGUCHI, R
HORN, GT
MULLIS, KB
ERLICH, HA
[J]. SCIENCE, 1988, 239 (4839) : 487 - 491
[6] TRAINOR KJ, 1990, BLOOD, V75, P2220
[7] DETECTION OF MINIMAL DISEASE IN HEMATOPOIETIC MALIGNANCIES OF THE B-CELL LINEAGE BY USING 3RD-COMPLEMENTARITY-DETERMINING REGION (CDR-III)-SPECIFIC PROBES
YAMADA, M
HUDSON, S
TOURNAY, O
BITTENBENDER, S
SHANE, SS
LANGE, B
TSUJIMOTO, Y
CATON, AJ
ROVERA, G
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (13) : 5123 - 5127
[8] PREFERENTIAL UTILIZATION OF SPECIFIC IMMUNOGLOBULIN HEAVY-CHAIN DIVERSITY AND JOINING SEGMENTS IN ADULT HUMAN PERIPHERAL-BLOOD LYMPHOCYTES-B
YAMADA, M
WASSERMAN, R
REICHARD, BA
SHANE, S
CATON, AJ
ROVERA, G
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 173 (02) : 395 - 407

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