RECEPTOR AFFINITY PURIFICATION OF A LIPID-BINDING ADHESIN FROM HELICOBACTER-PYLORI

被引：71

作者：

LINGWOOD, CA

WASFY, G

HAN, HM

HUESCA, M

机构：

[1] HOSP SICK CHILDREN,DEPT MICROBIOL,555 UNIV AVE,TORONTO M5G 1X8,ONTARIO,CANADA

[2] UNIV TORONTO,DEPT MICROBIOL,TORONTO M5S 1A1,ONTARIO,CANADA

[3] UNIV TORONTO,DEPT BIOCHEM & CLIN BIOCHEM,TORONTO M5S 1A1,ONTARIO,CANADA

来源：

INFECTION AND IMMUNITY | 1993年 / 61卷 / 06期

关键词：

D O I：

10.1128/IAI.61.6.2474-2478.1993

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Our previous work has shown that Helicobacter pylori specifically recognizes gangliotetraosyleeramide, gangliotriaosylceramide, and phosphatidylethanolamine in vitro. This binding specificity is shared by exoenzyme S from Pseudomonas aeruginosa, and monoclonal antibodies against this adhesin prevent the attachment of H. pylori to its lipid receptors. We now report the use of a novel, versatile affinity matrix to purify a 63-kDa exoenzyme S-like adhesin from H. pylori which is responsible for the lipid-binding specificity of this organism.

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页码：2474 / 2478

页数：5

相关论文

共 30 条

[1] RESPONSE OF CAMPYLOBACTER-PYLORIDIS TO ANTIBIOTICS, BISMUTH AND AN ACID-REDUCING AGENT INVITRO - AN ULTRASTRUCTURAL-STUDY [J].

ARMSTRONG, JA ;

WEE, SH ;

GOODWIN, CS ;

WILSON, DH .

JOURNAL OF MEDICAL MICROBIOLOGY, 1987, 24 (04) :343-350

[2] EVIDENCE FOR 2 CLASSES OF CARBOHYDRATE BINDING-SITES ON SELECTINS [J].

ASA, D ;

GANT, T ;

ODA, Y ;

BRANDLEY, BK .

GLYCOBIOLOGY, 1992, 2 (05) :395-399

[3] PSEUDOMONAS-AERUGINOSA EXOENZYME-S IS AN ADHESIN [J].

BAKER, NR ;

MINOR, V ;

DEAL, C ;

SHAHRABADI, MS ;

SIMPSON, DA ;

WOODS, DE .

INFECTION AND IMMUNITY, 1991, 59 (09) :2859-2863

[4] SELECTINS - A FAMILY OF ADHESION RECEPTORS [J].

BEVILACQUA, M ;

BUTCHER, E ;

FURIE, B ;

FURIE, B ;

GALLATIN, M ;

GIMBRONE, M ;

HARLAN, J ;

KISHIMOTO, K ;

LASKY, L ;

MCEVER, R ;

PAULSON, J ;

ROSEN, S ;

SEED, B ;

SIEGELMAN, M ;

SPRINGER, T ;

STOOLMAN, L ;

TEDDER, T ;

VARKI, A ;

WAGNER, D ;

WEISSMAN, I ;

ZIMMERMAN, G .

CELL, 1991, 67 (02) :233-233

[5] HELICOBACTER-PYLORI AND THE PATHOGENESIS OF GASTRODUODENAL INFLAMMATION [J].

BLASER, MJ .

JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (04) :626-633

[6]

DICK JD, 1990, ANNU REV MICROBIOL, V44, P249

[7]

DUNN BE, 1990, J BIOL CHEM, V265, P9464

[8] COMPARISON OF HELICOBACTER-PYLORI AND ATTACHING-EFFACING ESCHERICHIA-COLI ADHESION TO EUKARYOTIC CELLS [J].

DYTOC, M ;

GOLD, B ;

LOUIE, M ;

HUESCA, M ;

FEDORKO, L ;

CROWE, S ;

LINGWOOD, C ;

BRUNTON, J ;

SHERMAN, P .

INFECTION AND IMMUNITY, 1993, 61 (02) :448-456

[9] CLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION OF A GENE ENCODING AN ADHESIN SUBUNIT PROTEIN OF HELICOBACTER-PYLORI [J].

EVANS, DG ;

KARJALAINEN, TK ;

EVANS, DJ ;

GRAHAM, DY ;

LEE, CH .

JOURNAL OF BACTERIOLOGY, 1993, 175 (03) :674-683

[10] N-ACETYLNEURAMINYLLACTOSE-BINDING FIBRILLAR HEMAGGLUTININ OF CAMPYLOBACTER-PYLORI - A PUTATIVE COLONIZATION FACTOR ANTIGEN [J].

EVANS, DG ;

EVANS, DJ ;

MOULDS, JJ ;

GRAHAM, DY .

INFECTION AND IMMUNITY, 1988, 56 (11) :2896-2906