DEVELOPMENTAL-CHANGES IN MESSENGER-RNA ENCODING CARDIAC NA+/H+ EXCHANGER (NHE-1) IN RABBIT

被引：15

作者：

CHEN, FY

JARMAKANI, JM

VANDOP, C

机构：

[1] UNIV CALIF LOS ANGELES,DIV PEDIAT ENDOCRINOL,LOS ANGELES,CA 90095

[2] UNIV CALIF LOS ANGELES,DIV PEDIAT CARDIOL,LOS ANGELES,CA 90095

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 212卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.2063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The faster recovery of cardiac contractility in newborn rabbit hearts during acute acidosis compared to adult hearts correlates with greater cellular activity of the Na+/H+ exchanger. We quantified mRNA encoding Na+/H+ exchanger-1 (NHE-1) in rabbit ventricles of fetal (27 days gestation), newborn (2-5 days), and adult (>6 months) New Zealand white rabbits using reverse transcription-polymerase chain reaction (RT-PCR) and RNase protection assay, with GAPD mRNA as standard. Both RT-PCR and RNase protection assay revealed similar (p>0.05) cardiac levels of NHE-1 mRNA in fetal and newborn hearts, while NHE-1 mRNA in these hearts was 1.7x and 1.6x greater than in adult hearts by both RT-PCR and RNase protection assay. These developmental changes in NHE-1 mRNA correlate with the developmental changes in Na+/H+ activity in sarcolemmal vesicles purified from rabbit heart. (C) 1995 Academic Press,Inc.

引用

页码：960 / 967

页数：8

共 21 条

[1] TRANSCRIPTION OF THE DYSTROPHIN GENE IN HUMAN-MUSCLE AND NON-MUSCLE TISSUES [J].