IDENTITY DETERMINANTS OF ESCHERICHIA-COLI TRANSFER-RNA VAL

被引：43

作者：

TAMURA, K ^{[1
]}

HIMENO, H ^{[1
]}

ASAHARA, H ^{[1
]}

HASEGAWA, T ^{[1
]}

SHIMIZU, M ^{[1
]}

机构：

[1] INST SPACE & ASTRONAUT SCI, 3-1-1 YOSHINODAI, SAGAMIHARA, KANAGAWA 229, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 177卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91833-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to study the identity elements of valine tRNA, various transcripts of E. coli valine tRNA mutants were constructed. Both mutations at the second letter A35 of the anticodon and at the discriminator base A73 seriously damaged valine charging activity. Mutations at either the G3-C70 or U4-A69 base pairs in the acceptor stem also affected the activity. Only one nucleotide substitution of the second letter G35 of the anticodon with A35 brought an 18% valine charging activity into alanine tRNA, which acquired an almost full charging activity with valine by introducing an additional change at those two base pairs in the acceptor stem. These results indicate that the second letter A35 of the anticodon, discriminator base and acceptor stem are involved in recognition by valyl-tRNA synthetase. © 1991.

引用

页码：619 / 623

页数：5

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