RECOMMENDATIONS OF THE INTERNATIONAL COUNCIL FOR STANDARDIZATION IN HEMATOLOGY FOR ETHYLENEDIAMINETETRAACETIC ACID ANTICOAGULATION OF BLOOD FOR BLOOD-CELL COUNTING AND SIZING

被引:95
作者
ENGLAND, JM [1 ]
ROWAN, RM [1 ]
VANASSENDELFT, OW [1 ]
BULL, BS [1 ]
COULTER, W [1 ]
FUJIMOTO, K [1 ]
GRONER, W [1 ]
RICHARDSONJONES, A [1 ]
KLEE, G [1 ]
KOEPKE, JA [1 ]
LEWIS, SM [1 ]
MCLAREN, CE [1 ]
SHINTON, NK [1 ]
TATSUMI, N [1 ]
VERWILGHEN, RL [1 ]
机构
[1] NCID,CTR DIS CONTROL & PREVENT,PUBL HLTH SERV,DEPT HLTH & HUMAN SERV,1-2302 MS D-17,ATLANTA,GA 30333
关键词
ANTICOAGULATION; BLOOD CELL COUNT; EDTA;
D O I
10.1093/ajcp/100.4.371
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Of the three ethylenediaminetetraacetic acid (EDTA) salts used for anticoagulation of blood specimens for hematologic testing, potassium salts are the most readily soluble. Tripotassium EDTA is dispensed as a liquid and thus causes a slight dilution of the specimen. This salt also has been shown to affect the red blood cell size more at increased concentrations and on storage than the dipotassium salt. Therefore, dipotassium EDTA is recommended as the anticoagulant of choice in specimen collection for blood cell counting and sizing. The amount of dipotassium EDTA used is 1.5-2.2 mg (3.7-5.4 mumol) per milliliter of blood.
引用
收藏
页码:371 / 372
页数:2
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