ELECTROANALYTICAL X-RAY PHOTOELECTRON-SPECTROSCOPY INVESTIGATION ON GLUCOSE-OXIDASE ADSORBED ON PLATINUM

被引：27

作者：

DEBENEDETTO, GE

MALITESTA, C

ZAMBONIN, CG

机构：

来源：

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS | 1994年 / 90卷 / 11期

关键词：

D O I：

10.1039/ft9949001495

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Glucose oxidase has been adsorbed on Pt from a solution of the enzyme (0.5-15 mumol dm-3) in phosphate or acetate buffer (pH 3.9-8.0). The amperometric response to glucose of the modified electrodes so prepared has been evaluated. A spectroscopic (XPS) investigation of the system surface indicates that the enzyme molecules are stacked on the Pt surface, some directly interacting with the metal and the remaining part adsorbed on them. At the surface, the enzyme has a conformation in which its carbohydrate part tends to cover the proteic portion.

引用

页码：1495 / 1499

页数：5

共 31 条

[1] [Anonymous], 1992, HIGH RESOLUTION XPS, DOI 10.1021/ED070PA25.5
[2] METAL-IONS AND COMPLEXES AS MODULATORS OF PROTEIN INTERFACIAL ELECTRON-TRANSPORT AT GRAPHITE-ELECTRODES
ARMSTRONG, FA
COX, PA
HILL, HAO
LOWE, VJ
OLIVER, BN
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1987, 217 (02) : 331 - 366
[3] ELECTROCHEMICAL IMMOBILIZATION OF ENZYMES .2. GLUCOSE-OXIDASE IMMOBILIZED IN POLY-N-METHYLPYRROLE
BARTLETT, PN
WHITAKER, RG
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1987, 224 (1-2): : 37 - 48
[4] INVESTIGATION OF POLY(L-AMINO ACIDS) BY X-RAY PHOTOELECTRON-SPECTROSCOPY
BOMBEN, KD
DEV, SB
[J]. ANALYTICAL CHEMISTRY, 1988, 60 (14) : 1393 - 1397
[5] INTERFERENCE-FREE GLUCOSE SENSOR BASED ON GLUCOSE-OXIDASE IMMOBILIZED IN AN OVEROXIDIZED NONCONDUCTING POLYPYRROLE FILM
CENTONZE, D
GUERRIERI, A
MALITESTA, C
PALMISANO, F
ZAMBONIN, PG
[J]. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1992, 342 (09): : 729 - 733
[6] CENTONZE D, 1992, ANN CHIM-ROME, V82, P219
[7] CENTONZE D, 1994, IN PRESS ELECTROANAL
[8] EXPERIMENTAL AND THEORETICAL INVESTIGATION OF CORE LEVEL SPECTRA OF A SERIES OF AMINO-ACIDS, DIPEPTIDES AND POLYPEPTIDES
CLARK, DT
PEELING, J
COLLING, L
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 453 (02) : 533 - 545
[9] DILKS A, 1981, ELECTRON SPECTROSCOP, V4, pCH5
[10] USE OF X-RAY PHOTOELECTRON-SPECTROSCOPY TO STUDY PROTEIN ADSORPTION TO MICA SURFACES
FITZPATRICK, H
LUCKHAM, PF
ERIKSEN, S
HAMMOND, K
[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1992, 149 (01) : 1 - 9

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