COLD-SPECIFIC INDUCTION OF A DEHYDRIN GENE FAMILY MEMBER IN BARLEY

被引：49

作者：

VANZEE, K

CHEN, FQ

HAYES, PM

CLOSE, TJ

CHEN, THH

机构：

[1] OREGON STATE UNIV,DEPT HORT,CORVALLIS,OR 97331

[2] OREGON STATE UNIV,CTR GENE RES & BIOTECHNOL,CORVALLIS,OR 97331

[3] OREGON STATE UNIV,DEPT CROP & SOIL SCI,CORVALLIS,OR 97331

[4] UNIV CALIF RIVERSIDE,DEPT BOT & PLANT SCI,RIVERSIDE,CA 92521

来源：

PLANT PHYSIOLOGY | 1995年 / 108卷 / 03期

关键词：

D O I：

10.1104/pp.108.3.1233

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An interval on barley (Hordeum vulgare L.) chromosome 7 accounting for significant quantitative trait locus effects for winter hardiness were detected in a winter (Dicktoo) x spring (Morex) barley population (P.M. Hayes, T. Blake, T.H.H. Chen, S. Tragoonrung, F. Chen, A. Pan, and B. Liu [1993] Genome 36: 66-71). Two members of the barley dehydrin gene family, Dhn1 and Dhn2, were located within the region defining the winter hardiness quantitative trait locus effect (A. Pan, P.M. Hayes, F. Chen, T. Blake, T.H.H. Chen, T.T.S. Wright, I. Karsai, Z. Bedo [1994] Theor Appl Genet 89: 900-910). To investigate the possible role of Dhn1 and Dhn2 in winter hardiness, we examined the expression pattern of six barley dehydrin gene family members in shoot tissue in response to cold temperature. Incubation of 3-week-old barley plants at 2 degrees C resulted in a rapid induction of a single 86-kD polypeptide that was recognized by an antiserum against a peptide conserved in the dehydrin gene family. Northern blot analysis confirmed the induction of an mRNA corresponding to Dhn5. The expression patterns of cold-induced dehydrins in shoot tissue for Dicktoo and Morex were identical under the conditions studied, in spite of the known phenotypic differences in their winter hardiness. These results, together with the allelic structure of selected high- and low-survival lines, suggest that the Dicktoo alleles at the Dhn1 and Dhn2 may not be the primary determinants of winter hardiness in barley.

引用

页码：1233 / 1239

页数：7

共 38 条

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