INDUCTION OF THE PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR BY FENOFIBRATE IN RAT-LIVER

被引：104

作者：

GEBEL, T ^{[1
]}

ARAND, M ^{[1
]}

OESCH, F ^{[1
]}

机构：

[1] UNIV MAINZ,INST TOXICOL,OBERE ZAHLBACHERSTR 67,W-6500 MAINZ,GERMANY

来源：

FEBS LETTERS | 1992年 / 309卷 / 01期

关键词：

PPAR; FUSION PROTEIN; ANTIBODY; MESSENGER RNA; HYPOLIPIDEMIC COMPOUND;

D O I：

10.1016/0014-5793(92)80734-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The process of peroxisome proliferation in rodent liver by hypolipidemic compounds and related substances has recently been shown to be receptor-mediated. In the present study, we have examined the effect of oral administration of the strong peroxisome proliferator fenofibrate on the hepatic expression level of the peroxisome proliferator activated receptor (PPAR) in rats. Immunoblots of rat liver cytosols and nuclear extracts using antibodies raised against recombinant PPAR/beta-galactosidase fusion proteins revealed a pronounced increase in the amount of PPAR protein in response to fenofibrate treatment. This induction could also be confirmed at the level of RNA by Northern blotting. A time-course investigation showed a delayed accumulation of mRNA in response to the treatment, starting on day 2 after a latency period of at least one day. Thus, induction of the PPAR as a response to peroxisome proliferators represents one important dimension of the pleiotropic effects of peroxisome proliferators.

引用

页码：37 / 40

页数：4

共 18 条

[1]

IDENTIFICATION OF CYTOSOLIC PEROXISOME PROLIFERATOR BINDING-PROTEIN AS A MEMBER OF THE HEAT-SHOCK PROTEIN HSP70 FAMILY [J].

ALVARES, K ;

CARRILLO, A ;

YUAN, PM ;

KAWANO, H ;

MORIMOTO, RI ;

REDDY, JK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (14) :5293-5297

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[4]

CONTROL OF THE PEROXISOMAL BETA-OXIDATION PATHWAY BY A NOVEL FAMILY OF NUCLEAR HORMONE RECEPTORS [J].

DREYER, C ;

KREY, G ;

KELLER, H ;

GIVEL, F ;

HELFTENBEIN, G ;

WAHLI, W .

CELL, 1992, 68 (05) :879-887

[5]

FRIEDBERG T, 1991, METHOD ENZYMOL, P193

[6]

CYTOCHROME-P-450 INDUCTION BY CLOFIBRATE - PURIFICATION AND PROPERTIES OF A HEPATIC CYTOCHROME-P-450 RELATIVELY SPECIFIC FOR THE 12-HYDROXYLATION AND 11-HYDROXYLATION OF DODECANOIC ACID (LAURIC-ACID) [J].

GIBSON, GG ;

ORTON, TC ;

TAMBURINI, PP .

BIOCHEMICAL JOURNAL, 1982, 203 (01) :161-168

[7]

NATURE OF HEPATOMEGALIC EFFECT PRODUCED BY ETHYL-CHLOROPHENOXY-ISOBUTYRATE IN RAT [J].

HESS, R ;

STAUBLI, W ;

RIESS, W .

NATURE, 1965, 208 (5013) :856-+

[8]

ISSEMANN I, 1990, NATURE, V347, P645, DOI 10.1038/347645a0

[9]

PEROXISOME PROLIFERATOR-BINDING PROTEIN - IDENTIFICATION AND PARTIAL CHARACTERIZATION OF NAFENOPIN-BINDING, CLOFIBRIC ACID-BINDING, AND CIPROFIBRATE-BINDING PROTEINS FROM RAT-LIVER [J].

LALWANI, ND ;

ALVARES, K ;

REDDY, MK ;

REDDY, MN ;

PARIKH, I ;

REDDY, JK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (15) :5242-5246

[10]

LACK OF EVIDENCE FOR A HEPATIC PEROXISOME PROLIFERATOR RECEPTOR AND AN EXPLANATION FOR THE BINDING OF HYPOLIPEMIC DRUGS TO LIVER HOMOGENATES [J].

MILTON, MN ;

ELCOMBE, CR ;

KASS, GEN ;

GIBSON, GG .

BIOCHEMICAL PHARMACOLOGY, 1988, 37 (05) :793-798

← 1 2 →