MOBILIZATION OF A RECOMBINANT INCQ PLASMID BETWEEN BACTERIA ON AGAR AND IN SOIL VIA COTRANSFER OR RETROTRANSFER

被引:27
作者
SMIT, E
VENNE, D
VANELSAS, JD
机构
关键词
D O I
10.1128/AEM.59.7.2257-2263.1993
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Mobilization of a genetically engineered IncQ plasmid, pSKTG, was studied in vitro and in sterile and nonsterile soils. In biparental and triparental filter matings, the mobilization frequencies of pSKTG were identical, and the plasmid was mobilized only in the presence of self-transmissible plasmid RP4p. In sterile soil, mobilization was probably limited by reduced cell-to-cell contact, since the frequencies of mobilization were approximately 100-fold lower than the frequencies in the filter matings. The transfer frequency of pSKTG in sterile soil when RP4p was present in the same strain was about 100-fold higher than the transfer frequency when RP4p was present in a separate strain. In studies in natural soil, pSKTG was also found to be transferred to indigenous bacteria. However, natural mobilization by genetic elements present in the indigenous soil microflora could not be detected. In vitro studies of natural transfer suggested that such genetic elements occur in soil bacteria.
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页码:2257 / 2263
页数:7
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共 30 条
[1]  
BENDER CL, 1986, J BACTERIOL, V165, P535
[2]   DISTRIBUTION OF BACTERIAL PLASMIDS IN CLEAN AND POLLUTED SITES IN A SOUTH-WALES RIVER [J].
BURTON, NF ;
DAY, MJ ;
BULL, AT .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1982, 44 (05) :1026-1029
[3]   LARGE PLASMIDS GOVERNING MULTIPLE RESISTANCES TO HEAVY-METALS - A GENETIC APPROACH [J].
DIELS, L ;
SADOUK, A ;
MERGEAY, M .
TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY, 1989, 23 (1-4) :79-89
[4]  
FRY J, 1989, PROMISCUOUS PLASMIDS, P79
[5]  
FRY JC, 1990, BACTERIAL GENETICS IN NATURAL ENVIRONMENTS, P55
[6]   TRANSDUCTION OF ESCHERICHIA-COLI IN SOIL [J].
GERMIDA, JJ ;
KHACHATOURIANS, GG .
CANADIAN JOURNAL OF MICROBIOLOGY, 1988, 34 (02) :190-193
[7]  
HENSCHKE RB, 1990, CURR MICROBIOL, V20, P100
[8]   ISOLATION AND SCREENING OF PLASMIDS FROM THE EPILITHON WHICH MOBILIZE RECOMBINANT PLASMID PD10 [J].
HILL, KE ;
WEIGHTMAN, AJ ;
FRY, JC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (04) :1292-1300
[9]   RAPID PROCEDURE FOR DETECTION AND ISOLATION OF LARGE AND SMALL PLASMIDS [J].
KADO, CI ;
LIU, ST .
JOURNAL OF BACTERIOLOGY, 1981, 145 (03) :1365-1373
[10]   TRANSFORMATION OF BACILLUS-SUBTILIS BY DNA BOUND ON MONTMORILLONITE AND EFFECT OF DNASE ON THE TRANSFORMING ABILITY OF BOUND DNA [J].
KHANNA, M ;
STOTZKY, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (06) :1930-1939