A SINGLE POINT MUTATION IN THE DNA GYRASE-A PROTEIN GREATLY REDUCES BINDING OF FLUOROQUINOLONES TO THE GYRASE-DNA COMPLEX

被引：201

作者：

WILLMOTT, CJR ^{[1
]}

MAXWELL, A ^{[1
]}

机构：

[1] UNIV LEICESTER,DEPT BIOCHEM,LEICESTER LE1 7RH,ENGLAND

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1993年 / 37卷 / 01期

关键词：

D O I：

10.1128/AAC.37.1.126

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Binding of the quinolone drug norfloxacin to gyrase and DNA has been investigated. We have detected binding to gyrase-DNA complex but find no significant binding to either gyrase or DNA alone. Enzyme containing gyrase A protein with the mutation Ser-83 to Trp (conferring quinolone resistance) showed greatly reduced drug binding.

引用

页码：126 / 127

页数：2

共 16 条

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