RESPONSE OF A YEAST GLYCOGEN-SYNTHASE GENE TO STRESS

被引：43

作者：

NI, HT ^{[1
]}

LAPORTE, DC ^{[1
]}

机构：

[1] UNIV MINNESOTA,DEPT BIOCHEM,MINNEAPOLIS,MN 55455

来源：

MOLECULAR MICROBIOLOGY | 1995年 / 16卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.1995.tb02342.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the yeast Saccharomyces cerevisiae, glycogen synthase is encoded by two genes: GSY1 and GSY2. The activity of the enzymes increases as cultures enter the stationary phase of growth. Using a GSY2::lacZ fusion gene, we have demonstrated that the increase in glycogen synthase activity resulted, at least in part, from an increase in the level of the protein rather than simply from a change in its phosphorylation state. Northern blot analysis showed a parallel increase in the level of the GSY2 mRNA, which is consistent with transcriptional activation of GSY2. Deletion analysis identified three regions upstream of GSY2 which are involved in GSY2 expression: regions A (-390 to -347 relative to the start of translation), B (-252 to -209) and C (-209 to -167). Region A or C independently activated expression of GSY2. In contrast, region B alone yielded only modest expression. Expression of GSY2 is induced by growth to stationary phase, heat shock or nitrogen starvation. Response to these stressors is mediated by elements within regions A and C. These elements appear to be related to the stress-response elements found in other stress-responsive genes.

引用

页码：1197 / 1205

页数：9

共 34 条

[1]

Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Smith J.A., Seidman J.G., Struhl K., Current Protocols in Molecular Biology, (1987)

[2]

Becker J.-U., Mechanisms of regulation of glycogen phosphorylase activity in Saccharomyces carlsbergensis, J Gen Micro, 128, pp. 447-454, (1982)

[3]

Belazzi T., Wagner A., Wieser R., Schanz M., Adam G., Hartig A., Ruis H., Negative regulation of transcription of the Saccharomyces cerevisiae catalase T CTT1 gene by cAMP is mediated by a positive control element, EMBO J, 10, pp. 585-592, (1991)

[4]

Bradford M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding, Anal Biochem, 72, pp. 248-254, (1976)

[5]

Cannon J.F., Tatchell K., Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMF‐dependent protein kinase, Mol Cell Biol, 7, pp. 2653-2663, (1987)

[6]

Casadaban M.J., Martinez-Arlas A., Shapira S.K., Chou J., pp. 293-309, (1983)

[7]

Farkas I., Hardy T.A., DePaoli-Roach A.A., Roach P.J., Isolation of the GSY1 gene encoding yeast glycogen synthase and evidence for the existence of a second gene, J Biol Chem, 265, pp. 20879-20886, (1990)

[8]

Farkas I., Hardy T.A., Goebl M.G., Roach P.J., Two glycogen aynthase isoforms In Saccharomyces cerevisiae are coded by distinct genes that are differentially controlled, J Biol Chem, 266, pp. 15602-15607, (1991)

[9]

Fosset U., Muir L.W., Nielsen L.D., Fischer E.H., Purification and properties of yeast glycogen phosphorylase a and b, Biochemistry, 10, pp. 5105-5113, (1971)

[10]

Francois J., Villanueva M.E., Hers H.-G., The control of glycogen metabolism in yeast. I. Interconversion in vivo of glycogen synthase and glycogen phosphorylase induced by glucose, a nitrogen source or uncouplers, Eur J Biochem, 174, pp. 551-559, (1988)

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