EVALUATION OF PYRIMIDO[5,4-D]PYRIMIDINE DERIVATIVES AS PEROXYOXALATE CHEMILUMINESCENCE REAGENTS USING A FLOW-INJECTION SYSTEM

Eighteen kinds of pyrimido[5,4‐d]pyrimidines together with several commercially available fluorescent compounds such as perylene, Rhodamine B, etc., were evaluated as the reagents for a peroxyoxalate chemiluminescence (CL) detection system by using a flow injection method. The peroxyoxalate CL reaction employed consists of bis(2,4,6‐trichlorophenyl)oxalate, hydrogen peroxide, triethylamine, and a fluorophore. Under the conditions used, 2,6‐bis[di‐(2‐hydroxyethyl)amino]‐4,8‐dipiperidinopyrimido[5,4‐d]pyrimridine (Dipyridamole) and 2,4,6,8‐tetrathiomorpholinopyrimido[5,4‐d]pyrimidine (1i) gave very intense chemiluminescence intensities which were larger than those of any other commercially available fluorescent compounds tested (e.g., 10 times larger than that of perylene). Copyright © 1990 John Wiley & Sons, Ltd.