THE USE OF TOLUIDINE BLUE FOR INSITU DETECTION OF MICROORGANISMS IN FOODS

被引:18
作者
DODD, CER
WAITES, WM
机构
[1] Department of Applied Biochemistry and Food Science, Faculty of Agricultural and Food Sciences, University of Nottingham, Loughborough, Leicestershire, LE12 5RD, Sutton Bonington
关键词
D O I
10.1111/j.1472-765X.1991.tb00613.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The use of toluidine blue to stain cryosections of food samples to detect sites of microbial growth was examined. The method successfully detected colonies and single cells of both yeasts and bacteria at magnifications of x 400 or below in the majority of foods studied. The method demonstrates great potential for studying the micro-environments in which micro-organisms grow.
引用
收藏
页码:220 / 223
页数:4
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