INFECTIOUS VIRUS-REPLICATION IN PAPILLOMAS INDUCED BY MOLECULARLY CLONED COTTONTAIL RABBIT PAPILLOMAVIRUS DNA

被引：28

作者：

BRANDSMA, JL ^{[1
]}

XIAO, W ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT EPIDEMIOL & PUBL HLTH,NEW HAVEN,CT 06510

来源：

JOURNAL OF VIROLOGY | 1993年 / 67卷 / 01期

关键词：

D O I：

10.1128/JVI.67.1.567-571.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The ability to obtain infectious papillomavirus virions from molecularly cloned DNA has not been previously reported. We demonstrate here that viral genomes isolated from a recombinant DNA clone of cottontail rabbit papillomavirus (CRPV) gave rise to infectious virus when inoculated into cottontail rabbit skin. Replication occurred in papillomas that formed at inoculation sites. Extract of a DNA-induced papilloma was serially passaged to naive rabbits with high efficiency. Complete virus was fractionated on cesium chloride density gradients, and papillomavirus particles were visualized by electron microscopy. CRPV DNA isolated from virions contained DNA sequence polymorphisms that are characteristic of the input CRPV-WA strain of virus, thereby proving that the newly generated virus originated from the molecularly cloned viral genome. These findings indicate that this will be a useful system in which to perform genetic analysis of viral gene functions involved in replication.

引用

页码：567 / 571

页数：5

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