ANALYSIS OF RAT URINE FOR METABOLITES OF PYRILAMINE VIA HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY THERMOSPRAY MASS-SPECTROMETRY AND TANDEM MASS-SPECTROMETRY

被引：25

作者：

KORFMACHER, WA

FREEMAN, JP

GETEK, TA

BLOOM, J

HOLDER, CL

机构：

[1] US Food and Drug Administration, National Center for Toxicological Research, Jefferson, Arkansas

[2] Department of Chemistry, University of Puerto Rico., Rio Piedras

来源：

BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY | 1990年 / 19卷 / 03期

关键词：

D O I：

10.1002/bms.1200190317

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Combined high‐performance liquid chromatography/thermospray mass spectrometry (HPLC/TSMS) and HPLC/thermospray tandem mass spectrometry (HPLC/TSMS/MS) were utilized for the analysis of rat urine for metabolites of pyrilamine. The sample was analyzed via HPLC/TSMS/MS in the parent ion mode in order to identify potential metabolites of pyrilamine. Then HPLC/TSMS/MS analysis in the daughter ion mode was performed to provide additional analytical selectivity plus enhanced fragmentation of suspected protonated molecules. By this methodology, suspected pyrilamine metabolites were confirmed to be in the sample and several novel metabolites of pyrilamine were discovered and tentatively identified. Copyright © 1990 John Wiley & Sons, Ltd.

引用

页码：191 / 201

页数：11

共 31 条

[1] RAPID STRUCTURAL-ANALYSIS OF THE INVITRO AND INVIVO METABOLISM OF SK-AND-F-95448 BY THE COMBINED USE OF THERMOSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY AND LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY [J].