CLONING AND SEQUENCE OF THE SCS3 GENE WHICH IS REQUIRED FOR INOSITOL PROTOTROPHY IN SACCHAROMYCES-CEREVISIAE

被引：29

作者：

HOSAKA, K

NIKAWA, J

KODAKI, T

ISHIZU, H

YAMASHITA, S

机构：

[1] Department of Biochemistry, Gunma University School of Medicine, Maebashi

来源：

JOURNAL OF BIOCHEMISTRY | 1994年 / 116卷 / 06期

关键词：

INOSITOL AUXOTROPHY; INOSITOL SYNTHESIS; SACCHAROMYCES CEREVISIAE;

D O I：

10.1093/oxfordjournals.jbchem.a124681

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The SCS3 gene of Saccharomyces cerevisiae was cloned by functional complementation, using a conditional mutant exhibiting myo-inositol auxotrophy in the presence of choline, and sequenced. The sequence contained an open reading frame capable of encoding 380 amino acids with a calculated molecular weight of 42,734. Disruption of the SCS3 locus caused myo-inositol auxotrophy. The gene appeared to be involved in the synthesis of inositol phospholipids from inositol but not in the control of inositol synthesis.

引用

页码：1317 / 1321

页数：5

共 39 条

[1] A SUPPRESSOR OF A HIS4 TRANSCRIPTIONAL DEFECT ENCODES A PROTEIN WITH HOMOLOGY TO THE CATALYTIC SUBUNIT OF PROTEIN PHOSPHATASES [J].