ISOLATION AND CHARACTERIZATION OF THE CANINE THYROGLOBULIN GENE PROMOTER REGION

被引：15

作者：

DONDA, A

VASSART, G

CHRISTOPHE, D

机构：

[1] UNIV LIBRE BRUXELLES,INST INTERDISCIPLINARY RES,CAMPUS ERASME,BAT C,808 ROUTE LENNIK,B-1070 BRUSSELS,BELGIUM

[2] UNIV LIBRE BRUXELLES,DEPT MED GENET,B-1070 BRUSSELS,BELGIUM

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1090卷 / 02期

关键词：

THYROGLOBULIN GENE; DNAASE-I FOOTPRINTING; GENE EXPRESSION; (CANINE);

D O I：

10.1016/0167-4781(91)90107-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 5' flanking sequences from the canine thyroglobulin gene were isolated by homology screening with the evolutionary conserved sequence from the bovine thyroglobulin promoter and sequenced. Transient expression in primary cultured dog thyrocytes demonstrated that the canine clone contains a functional promoter inducible by CAMP. DNAse I footprinting assays showed that the thyroid-specific transcription factor TTF-1, purified from bovine thyroid, also recognizes the canine thyroglobulin promoter. Similar footprints were obtained with crude nuclear extracts from primary cultured dog thyrocytes.

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页码：235 / 237

页数：3

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