TUMOR-NECROSIS-FACTOR INVOLVEMENT IN MYOCARDIAL ISCHEMIA-REPERFUSION INJURY

被引：61

作者：

SQUADRITO, F ^{[1
]}

ALTAVILLA, D ^{[1
]}

ZINGARELLI, B ^{[1
]}

IOCULANO, M ^{[1
]}

CALAPAI, G ^{[1
]}

CAMPO, GM ^{[1
]}

MICELI, A ^{[1
]}

CAPUTI, AP ^{[1
]}

机构：

[1] UNIV MESSINA,SCH BIOL SCI,CHAIR PHARMACOL,I-98100 MESSINA,ITALY

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 1993年 / 237卷 / 2-3期

关键词：

TNF-ALPHA (TUMOR NECROSIS FACTOR-ALPHA); ANTITUMOR NECROSIS FACTOR-ALPHA ANTIBODIES; ISCHEMIA-REPERFUSION INJURY;

D O I：

10.1016/0014-2999(93)90272-J

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The role of tumor necrosis factor-alpha was investigated in an anaesthetized rat model of coronary artery ligation (60 min) and reperfusion (MI/R). Sham-occluded rats (sham MI/R) were used as controls. Survival rate, myocardial necrosis, myocardial myeloperoxidase activity, serum creatinine kinase activity and serum and macrophage tumor necrosis factor-alpha were studied. Ischaemia-reperfusion injury significantly reduced survival rate (45%), produced marked myocardial injury, increased serum creatinine kinase activity and increased myocardial myeloperoxidase activity in the area-at-risk and in the necrotic area. Serum tumor necrosis factor-alpha was undetectable during the occlusion period, but increased significantly upon release of the coronary artery. At the end of reperfusion, macrophage tumor necrosis factor-alpha was also increased. Passive immunization with a hyperimmune serum containing antibodies against murine tumor necrosis factor-alpha significantly increased survival rate (80%), lowered myocardial necrosis, reduced the increase in serum creatinine kinase activity and decreased myeloperoxidase activity in the area-at-risk and in the necrotic area. These data are consistent with an involvement of tumor necrosis factor-alpha in myocardial ischaemia-reperfusion injury.

引用

页码：223 / 230

页数：8

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