EVALUATION OF A NEUTRAL HYDROPHILIC COATED CAPILLARY FOR CAPILLARY ZONE ELECTROPHORETIC SEPARATION OF PROTEINS

A neutral, hydrophilic coated capillary with negligible electroosmotic flow was characterized as to migration time reproducibility and separation efficiency for protein separation by capillary zone electrophoresis. Consecutive runs (over 200 runs) of the basic proteins at pH 6.0 yielded excellent migration time reproducibility (< 2% R.S.D.) and high separation efficiency (ca. 3-5.10(5) plates/m). The acidic proteins were separated at pH 8.0 under the reversed polarity (cathode at the injection end), and excellent migration time reproducibility of less than 3% was achieved. Separation of egg white proteins at pH 3.0 showed migration time reproducibility of less than 0.5% R.S.D. (n = 36) for lysozyme, conalbumin and ovalbumin.