RECEPTOR-MEDIATED MN2+ INFLUX IN RAT HEPATOCYTES - COMPARISON OF CELLS LOADED WITH FURA-2 ESTER AND CELLS MICROINJECTED WITH FURA-2 SALT

被引：25

作者：

KASS, GEN ^{[1
]}

WEBB, DL ^{[1
]}

CHOW, SC ^{[1
]}

LLOPIS, J ^{[1
]}

BERGGREN, PO ^{[1
]}

机构：

[1] KAROLINSKA INST, ROLF LUFT CTR DIABET RES, DEPT ENDOCRINOL, S-17177 STOCKHOLM, SWEDEN

来源：

BIOCHEMICAL JOURNAL | 1994年 / 302卷

关键词：

D O I：

10.1042/bj3020005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+. Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl)hydroquinone or thapsigargin did not. The pattern of Fura-2 quenching by Mn2+ only correlated with the movement of Mn2+ across the plasma membrane.

引用

页码：5 / 9

页数：5

共 35 条

[1]

ARENDS MJ, 1991, INT REV EXP PATHOL, V32, P223

[2] INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].

BERRIDGE, MJ .

NATURE, 1993, 361 (6410) :315-325

[3]

BIRD GS, 1993, J BIOL CHEM, V268, P21486

[4]

BYRON KL, 1992, J BIOL CHEM, V267, P108

[5] 2 INDEPENDENTLY REGULATED CA2+ ENTRY MECHANISMS COEXIST IN JURKAT T-CELLS DURING T-CELL RECEPTOR ANTIGEN ACTIVATION [J].

CHOW, SC ;

KASS, GEN ;

ORRENIUS, S .

BIOCHEMICAL JOURNAL, 1993, 293 :395-398

[6]

CLEMENTI E, 1992, J BIOL CHEM, V267, P2164

[7] THE CONTROL OF APOPTOSIS IN MAMMALIAN-CELLS [J].

COLLINS, MKL ;

RIVAS, AL .

TRENDS IN BIOCHEMICAL SCIENCES, 1993, 18 (08) :307-309

[8] INTRACELLULAR CALCIUM MOBILIZATION BY INOSITOL 1,4,5-TRISPHOSPHATE - INTRACELLULAR MOVEMENTS AND COMPARTMENTALIZATION [J].

CONNOR, JA .

CELL CALCIUM, 1993, 14 (03) :185-200

[9]

ELLIS RE, 1991, ANNU REV CELL BIOL, V7, P663, DOI 10.1146/annurev.cb.07.110191.003311

[10]

ELY JA, 1991, J BIOL CHEM, V266, P18635

← 1 2 3 4 →