DEVELOPMENTAL AND HORMONAL-REGULATION OF A MOUSE MAMMARY-TUMOR VIRUS GLYCOPROTEIN IN NORMAL MOUSE MAMMARY EPITHELIUM

被引:12
作者
BOLANDER, FF
BLACKSTONE, ME
机构
关键词
D O I
10.1677/jme.0.0040101
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
A radioimmunoassay was developed and validated for the major glycoprotein (gp58) of the mouse mammary tumour virus (MMTV). Using this assay, the expression of gp58 during pregnancy and lactation was found to parallel that for MMTV RNA. In particular, there was a very rapid induction in late pregnancy and a decline in late lactation, although some residual expression persisted well into involution. In cultures of normal mouse mammary tissue, induction of gp58 occurred after a 24-h lag period and began to reach a plateau after 3 days. Both the insulin and prolactin dose-response curves for gp58 resembled those for MMTV RNA; in contrast, the effects of steroid hormones on pg58 and MMTV RNA were disparate, Although progesterone stimulated the RNA, it only slightly increased gp 58 levels; however, the presence of cortisol greatly augmented this stimulation, despite the inability of cortisol to induce RNA at physiological concentrations. These results suggest that insulin, prolactin and progesterone act primarily at the level of RNA accumulation in normal mammary epithelium, while cortisol affects some more distal event.
引用
收藏
页码:101 / 106
页数:6
相关论文
共 33 条
[1]   COMMON SURFACE-RECEPTORS ON BOTH MOUSE AND RAT-CELLS DISTINGUISH DIFFERENT CLASSES OF MOUSE MAMMARY-TUMOR VIRUSES [J].
ALTROCK, BW ;
ARTHUR, LO ;
MASSEY, RJ ;
SCHOCHETMAN, G .
VIROLOGY, 1981, 109 (02) :257-266
[2]   PHORBOL ESTER-MEDIATED STIMULATION OF THE SYNTHESIS OF MOUSE MAMMARY-TUMOR VIRUS [J].
ARYA, SK .
NATURE, 1980, 284 (5751) :71-72
[3]   STIMULATION OF LACTOSE SYNTHETASE-ACTIVITY AND CASEIN SYNTHESIS IN MOUSE MAMMARY EXPLANTS BY ESTRADIOL [J].
BOLANDER, FF ;
TOPPER, YJ .
ENDOCRINOLOGY, 1980, 106 (02) :490-495
[4]   GROWTH-HORMONE COVALENTLY BOUND TO SEPHAROSE OR GLASS - ANALYSIS OF LIGAND RELEASE RATES AND CHARACTERIZATION OF SOLUBLE RADIOLABELED PRODUCTS [J].
BOLANDER, FF ;
FELLOWS, RE .
BIOCHEMISTRY, 1975, 14 (13) :2938-2943
[5]  
BOLANDER FF, 1989, MOL ENDOCRINOL, P255
[6]   THE ROLE OF SEX STEROIDS IN THE EXPRESSION OF MMTV IN THE NORMAL MOUSE MAMMARY-GLAND [J].
BRADHAM, BM ;
BOLANDER, FF .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 159 (03) :1020-1025
[8]   THE HORMONE REGULATORY ELEMENT OF MOUSE MAMMARY-TUMOR VIRUS MEDIATES PROGESTERONE INDUCTION [J].
CATO, ACB ;
MIKSICEK, R ;
SCHUTZ, G ;
ARNEMANN, J ;
BEATO, M .
EMBO JOURNAL, 1986, 5 (09) :2237-2240
[9]   ESSENTIAL ROLE OF INSULIN IN TRANSCRIPTION OF THE RAT 25,000 MOLECULAR-WEIGHT CASEIN GENE [J].
CHOMCZYNSKI, P ;
QASBA, P ;
TOPPER, YJ .
SCIENCE, 1984, 226 (4680) :1326-1328
[10]  
DICKSON C, 1983, CURR TOP MICROBIOL, V106, P1