EFFECTS OF TRANSFORMING GROWTH-FACTOR-BETA ON COLLAGEN GENE-EXPRESSION AND COLLAGEN-SYNTHESIS LEVEL IN MINERALIZING CULTURES OF OSTEOBLAST-LIKE CELL-LINE, MC3T3-E1

被引：12

作者：

SHIBATA, Y ^{[1
]}

ABIKO, Y ^{[1
]}

MORIYA, Y ^{[1
]}

YOSHIDA, W ^{[1
]}

TAKIGUCHI, H ^{[1
]}

机构：

[1] NIHON UNIV,SCH DENT MATSUDO,DEPT PHARMACOL,MATSUDO,CHIBA 271,JAPAN

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1993年 / 25卷 / 02期

关键词：

D O I：

10.1016/0020-711X(93)90012-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. High levels of type I collagen mRNA and [H-3]proline incorporation into collagenase digestable protein by MC3T3-E1 cells were detected during the first 7 days of culture, after which they declined. 2. Type I collagen gene expression was stimulated by TGF-beta in the early culture stage when the collagen gene expression was fully functioning. 3. However, these stimulatory effects disappeared at the differentiation stages. Although collagen gene expression was stimulated by TGF-beta (2.0 ng/ml) in early culture, collagen synthesis in medium was not. 4. This study shows that collagen synthesis and collagen gene expression were affected by the state of differentiation in MC3T3-E1 cells and that the rate of stimulation by TGF-beta in collagen gene expression decreased over time in culture.

引用

页码：239 / 245

页数：7

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