SEQUENCE VARIATION IN 2 IPAH GENES OF SHIGELLA-FLEXNERI 5 AND HOMOLOGY TO THE LRG-LIKE FAMILY OF PROTEINS

被引:51
作者
VENKATESAN, MM [1 ]
BUYSSE, JM [1 ]
HARTMAN, AB [1 ]
机构
[1] WALTER REED ARMY MED CTR,DEPT BIOL RES,DIV COMMUNICABLE DIS & IMMUNOL,WASHINGTON,DC 20307
关键词
D O I
10.1111/j.1365-2958.1991.tb02089.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oligonucleotide primers derived from the ipaH7.8 sequence have been used to determine the boundaries of DNA sequence homology among five ipaH genes on the invasion plasmid (pWR100) of Shigella flexneri 5, strain M9OT-W. The primary structure of IpaH4.s has been established from DNA sequence analysis. The first 197 amino acids in IpaH7.8 were replaced in IpaH4.5 by a unique set of 251 amino acids, generating two related proteins with variable and conserved sequences. The amino-terminal region of IpaH4.5 displayed an internal repeat structure, also seen in IpaH7.8, characteristic of members of the leucine-rich glycoprotein (LRG) family. The DNA sequences of ipaH2.5 and ipaH1.4 indicate that these genes are truncated versions of iPaH7.8. Western blot analysis of a lambda-gt11 ipaH recombinant (W7) subclone demonstrated that the antigenicity of IpaH7.8 resides outside the leucine-rich repetitive region.
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页码:2435 / 2445
页数:11
相关论文
共 39 条
[1]   IDENTIFICATION OF ICSA, A PLASMID LOCUS OF SHIGELLA-FLEXNERI THAT GOVERNS BACTERIAL INTRA-CELLULAR AND INTERCELLULAR SPREAD THROUGH INTERACTION WITH F-ACTIN [J].
BERNARDINI, ML ;
MOUNIER, J ;
DHAUTEVILLE, H ;
COQUISRONDON, M ;
SANSONETTI, PJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (10) :3867-3871
[2]   MOLECULAR-CLONING OF INVASION PLASMID ANTIGEN (IPA) GENES FROM SHIGELLA-FLEXNERI - ANALYSIS OF IPA GENE-PRODUCTS AND GENETIC-MAPPING [J].
BUYSSE, JM ;
STOVER, CK ;
OAKS, EV ;
VENKATESAN, M ;
KOPECKO, DJ .
JOURNAL OF BACTERIOLOGY, 1987, 169 (06) :2561-2569
[3]   MOLECULAR CHARACTERIZATION OF A TRANS-ACTING, POSITIVE EFFECTOR (IPAR) OF INVASION PLASMID ANTIGEN SYNTHESIS IN SHIGELLA-FLEXNERI SEROTYPE-5 [J].
BUYSSE, JM ;
VENKATESAN, M ;
MILLS, JA ;
OAKS, EV .
MICROBIAL PATHOGENESIS, 1990, 8 (03) :197-211
[4]   LOCAL AND SYSTEMIC ANTIBODY-RESPONSES TO SHIGELLA INFECTION IN RHESUS-MONKEYS [J].
DINARI, G ;
HALE, TL ;
AUSTIN, SW ;
FORMAL, SB .
JOURNAL OF INFECTIOUS DISEASES, 1987, 155 (05) :1065-1069
[5]   SEQUENCE AND MOLECULAR CHARACTERIZATION OF A MULTICOPY INVASION PLASMID ANTIGEN GENE, IPAH, OF SHIGELLA-FLEXNERI [J].
HARTMAN, AB ;
VENKATESAN, M ;
OAKS, EV ;
BUYSSE, JM .
JOURNAL OF BACTERIOLOGY, 1990, 172 (04) :1905-1915
[6]   THE TOLL GENE OF DROSOPHILA, REQUIRED FOR DORSAL-VENTRAL EMBRYONIC POLARITY, APPEARS TO ENCODE A TRANSMEMBRANE PROTEIN [J].
HASHIMOTO, C ;
HUDSON, KL ;
ANDERSON, KV .
CELL, 1988, 52 (02) :269-279
[7]   HUMAN-PLATELET GLYCOPROTEIN-IX - AN ADHESIVE PROTOTYPE OF LEUCINE-RICH GLYCOPROTEINS WITH FLANK-CENTER-FLANK STRUCTURES [J].
HICKEY, MJ ;
WILLIAMS, SA ;
ROTH, GJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (17) :6773-6777
[8]   AMINO-ACID SEQUENCE OF THE RIBONUCLEASE INHIBITOR FROM PORCINE LIVER REVEALS THE PRESENCE OF LEUCINE-RICH REPEATS [J].
HOFSTEENGE, J ;
KIEFFER, B ;
MATTHIES, R ;
HEMMINGS, BA ;
STONE, SR .
BIOCHEMISTRY, 1988, 27 (23) :8537-8544
[9]   DNA-SEQUENCE AND CHARACTERIZATION OF THE S-CEREVISIAE GENE ENCODING ADENYLATE-CYCLASE [J].
KATAOKA, T ;
BROEK, D ;
WIGLER, M .
CELL, 1985, 43 (02) :493-505
[10]  
KOPECKO DJ, 1988, ENTERIC INFECTION ME, P41