A SEQUENCE AT THE INSIDE END OF IS50 DOWN REGULATES TRANSPOSITION

被引：3

作者：

DODSON, KW

BERG, DE

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT MOLEC MICROBIOL,BOX 8230,660 S EUCLID AVE,ST LOUIS,MO 63110

[2] WASHINGTON UNIV,SCH MED,DEPT GENET,ST LOUIS,MO 63110

来源：

PLASMID | 1991年 / 25卷 / 02期

关键词：

D O I：

10.1016/0147-619X(91)90028-U

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Deletion analysis has shown that the segment at the IS50 inside (I) end that is needed for efficient transposition is ~19 bp long. Dam methylation at two 5′ GATC sequences within this segment decreases I-end transposition activity. A third 5′ GATC sequence is present at bp 21-24 of the I end. The comparisons presented here show that extension of the I end from 19 to 24 bp decreases its transposition activity in dam cells 5- to 50-fold, depending on the overall transposon structure. © 1991.

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页码：145 / 148

页数：4

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