A NOVEL SULFATASE FROM PSEUDOMONAS-TESTOSTERONI HYDROLYZING LITHOCHOLIC ACID SULFATE

被引：9

作者：

TAZUKE, Y

MATSUDA, K

OKADA, S

TSUKADA, Y

机构：

[1] Kyoto Research Laboratories, Marukin Shoyu Co., Ltd., Uji, Kyoto 611, 27 Monnomae, Todo

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1992年 / 56卷 / 10期

关键词：

D O I：

10.1271/bbb.56.1584

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pseudomonas testosteroni ATCC 11996 was found to produce a novel bile acid sulfate sulfatase that hydrolyzes the sulfate ester bond in lithocholic acid sulfate (LCA-S). The enzyme synthesis was induced by several kinds of bile acids including LCA-S. Mn2+ functioned as an essential component for the enzyme synthesis and SO42- suppressed it. This sulfatase hydrolyzes LCA-S to isolithocholic acid and sulfuric acid with inversion of alpha- to beta-configuration of the hydroxyl group at the third position of lithocholic acid.

引用

页码：1584 / 1588

页数：5

共 14 条

[1] ISOLATION OF A BILE-SALT SULFATASE-PRODUCING CLOSTRIDIUM STRAIN FROM RAT INTESTINAL MICROFLORA [J].