A NEW SELENOPROTEIN FOUND IN THE GLANDULAR EPITHELIAL-CELLS OF THE RAT PROSTATE

An inverse relationship between the Se status and the incidence of prostate cancer suggests a significant role of Se in this organ. After labeling of rats with Se-75 and sodium dodecyl sulfate-polyacrylamide gel electrophoresis a strongly labeled prostatic 15-kDa protein band was found which was equally distributed among the different lobes. It was localized in the epithelial cells of isolated acini but did not appear in the prostatic secretion. By two-dimensional electrophoresis the band was resolved into three spots with pI-values around 4.5. The most strongly labeled spot stemmed from a cytosolic selenoprotein with an apparent native molecular mass of about 300 kDa which contained Se in the form of selenocysteine. The fact that with insufficient Se intake the element is preferentially incorporated into this compound as compared with glutathione peroxidase implies an important function of this newly found prostatic epithelial selenoprotein (PES). (C) 1995 Academic Press, Inc.