COMPARISON OF MOUSE BIOASSAY, HPLC AND ENZYME-IMMUNOASSAY METHODS FOR DETERMINING DIARRHETIC SHELLFISH POISONING TOXINS IN MUSSELS

被引：30

作者：

DRAISCI, R

CROCI, L

GIANNETTI, L

COZZI, L

LUCENTINI, L

DEMEDICI, D

STACCHINI, A

机构：

[1] IST SUPER SANITA,REPARTO ASSITIVI CONTAMINANTI & ALIMENTI CONSERVA,I-00161 ROME,ITALY

[2] IST SUPER SANITA,REPARTO IGIENE TECNOL ALIMENTARI,ALIMENTI LAB,I-00161 ROME,ITALY

来源：

TOXICON | 1994年 / 32卷 / 11期

关键词：

D O I：

10.1016/0041-0101(94)90410-3

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Mussel specimens (Mytilus galloprovincialis) collected from two different areas of the Adriatic Sea were analysed for diarrhoetic shellfish poisoning (DSP) toxin by three methods: mouse bioassay, the DSP Check enzyme immunoassay kit, and high-performance liquid chromatography (HPLC). The results obtained confirm that Yasumoto's mouse bioassay, capable of detecting all the components of the DSP group, is still necessary to determine the wholesomeness of the product. The ELISA method has not always given quantitatively reliable results. The HPLC method is advantageous in terms of sensitivity, accuracy, specificity and rapidity. However, its application is limited so far to the determination of okadaic acid in mussels.

引用

页码：1379 / 1384

页数：6

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