BOTH DR AND MT CLASS-II HLA MOLECULES MAY RESTRICT PROLIFERATIVE LYMPHOCYTE-T RESPONSES TO ANTIGEN

Antigen-primed human T-cell blasts may be separated from alloreactive T cells on Percoll gradients. By this method, HLA restriction of antigen-specific proliferative T-cell responses may be studied, using allogeneic antigen-presenting cells carrying foreign D/DR antigens. The D/DR molecules as such are major restriction elements in the T-cell response to herpes simplex virus (HSV) and purified protein derivative. Evidence is presented that other Class II HLA molecules, the MT molecules, may also function as restriction elements for the HSV response.