PRODUCTION OF MONOCLONAL-ANTIBODIES SPECIFIC TO MAJOR ALLERGENS OF CRYPTOMERIA-JAPONICA POLLEN

被引：3

作者：

HONDA, K

SHIRAE, H

MIWA, K

MATSUSHITA, S

MAKISHIMA, K

NISHIMURA, Y

SASAZUKI, T

机构：

[1] UNIV OCCUPAT & ENVIRONM HLTH, SCH MORPHOL, DEPT OTORHINOLARYNGOL, KITAKYUSHU, FUKUOKA 807, JAPAN

[2] AJINOMOTO RES INST, KAWASAKI, FUKUOKA 210, JAPAN

[3] KYUSHU UNIV, MED INST BIOREGULAT, DEPT GENET, FUKUOKA 812, JAPAN

来源：

HYBRIDOMA | 1992年 / 11卷 / 03期

关键词：

D O I：

10.1089/hyb.1992.11.361

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoclonal antibodies (MAbs) directed against Cryptomeria japonica pollen antigen (CPAg) were prepared. CPAg was precipitated with these MAbs and both MAbs CPA7 (IgG2a) and CPA9 (IgG1) recognized two major glycoproteins (m = 41kDa and 46 kDa) of CPAg. MAb CPA7 or CPA9 was coupled to CNBr activated sepharose and affinity purification of the major allergen of CPAg from the crude extract was performed. The affinity purified CPAg bound IgE antibody present in patients with Cedar pollinosis.

引用

页码：361 / 366

页数：6

共 8 条

[1]

KENNETT RH, 1980, MONOCLONAL ANTIBODIE

[2]

MATSUSHITA S, 1987, J IMMUNOL, V138, P109

[3] STIMULATION OF PRIMARY INVITRO IGE ANTIBODY-RESPONSES IN CULTURES OF HUMAN PERIPHERAL MONONUCLEAR-CELLS [J].