SENSITIVE ENZYME-IMMUNOASSAY FOR HUMAN MN SUPEROXIDE-DISMUTASE

被引：24

作者：

KUROBE, N

INAGAKI, T

KATO, K

机构：

[1] AICHI PREFECTURAL COLONY, INST DEV RES, DEPT BIOCHEM, KASUGAI, AICHI 48003, JAPAN

[2] GERIATR HOSP, DEPT INTERNAL MED, NAGOYA, JAPAN

来源：

CLINICA CHIMICA ACTA | 1990年 / 192卷 / 03期

关键词：

Alzheimer's disease; Brain; Immunoassay; Superoxide dismutase;

D O I：

10.1016/0009-8981(90)90219-I

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A sensitive sandwich-type enzyme immunoassay for measurement of human Mn Superoxide dismutase (Mn SOD) was developed using purified antibodies specific to Mn SOD. The antisera were raised in rabbits by injecting Mn SOD purified from human liver. The antibody IgG, purified by the use of Mn SOD-coupled Sepharose, showed a single band on the immunoblotting test with a crude liver extract. The assay system consisted of polystyrene balls with immobilized monospecific antibody F(ab')2 fragments and the same antibody Fab' fragments labeled with β-D-galactosidase from Escherichia coli. The assay was highly sensitive and the minimum detection limit was 1 pg human Mn SOD/assay tube. Serum Mn SOD concentrations of healthy adults (77.5 ± 18.0 ng/ml (1 SD), n = 120, 16-64 yr old) were not related to age or sex. Immunoreactive Mn SOD was detectable in most tissues examined except for erythrocytes. The concentrations of immunoreactive Mn SOD and Cu/Zn SOD in the cerebral cortex were not different among the patients with Alzheimer's disease, and the age matched and young patients without neurological disorders. © 1990.

引用

页码：171 / 180

页数：10

共 20 条

[1] A RADIOIMMUNOASSAY FOR MANGANESE CONTAINING SUPEROXIDE-DISMUTASE [J].