FULLY ENZYMATIC INULIN DETERMINATION IN SMALL VOLUME SAMPLES WITHOUT DEPROTEINIZATION

被引：75

作者：

KUEHNLE, HF

SCHMIDT, FH

VONDAHL, K

机构：

来源：

NEPHRON | 1992年 / 62卷 / 01期

关键词：

INULIN; INULINASE; INULIN DETERMINATION; INULIN CLEARANCE;

D O I：

10.1159/000187006

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

A fully enzymatic method for the determination of inulin in serum or plasma without deproteinization is described. The assay is carried out by means of a fructose determination after hydrolysis of inulin via inulinase and simultaneous oxidation of the native glucose using glucose oxidase and H2O2. This time-saving procedure of high specificy, sensitivity and accuracy requires only small sample volumes. The usefulness of the method is shown by analysis of blood levels in humans and rabbits after an intravenous administration of Inutest(R).

引用

页码：104 / 107

页数：4

共 9 条

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DAVIDSON WD, 1963, J LAB CLIN MED, V62, P351

[2]

DIGREGORIO GJ, 1971, DRILLS PHARM MED, P1851

[3] ENZYMATIC-HYDROLYSIS OF PLANT-EXTRACTS CONTAINING INULIN [J].