RAPID METHODS FOR ISOLATION OF HUMAN-PLASMA FIBRONECTIN

Simplified procedures were developed for isolation of human plasma fibronectin by affinity chromatography on gelatin-agarose. In 1 method, fibronectin was eluted with 3 M urea and this reagent was quickly removed by adsorbing the protein onto heparinagarose, followed by 0.4 M NaCl elution. In a shorter process, fibronectin was eluted from gelatin-agarose simply by decreasing the buffer pH below 6. After lyophilization, the purified protein was readily dissolved in water. The fraction not adsorbed to gelatin was used to purify other proteins, including factor VIII whose procoagulant activity was quantitatively recovered.