TETRAPLEX FORMATION OF A GUANINE-CONTAINING NONAMERIC DNA FRAGMENT

被引：111

作者：

JIN, R ^{[1
]}

BRESLAUER, KJ ^{[1
]}

JONES, RA ^{[1
]}

GAFFNEY, BL ^{[1
]}

机构：

[1] RUTGERS STATE UNIV,DEPT CHEM,PISCATAWAY,NJ 08855

来源：

SCIENCE | 1990年 / 250卷 / 4980期

关键词：

D O I：

10.1126/science.2237404

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A combination of spectroscopic and calorimetric techniques has been used to characterize the structures formed by a family of short, guanine-containing DNA single strands of the form d[GGTTXTTGG], X=A, C, G, T. In 1 molar NaCl at low temperatures, these molecules do not behave like single strands, but rather exhibit properties consistent with tetraplex formation. The standard state enthalpies, entropies, and free energies for formation of each tetraplex have been measured, as have preliminary nuclear magnetic resonance (NMR) spectra. In 1 molar KCl, the melting behavior of the structure or structures is more complex than in 1 molar NaCl. This observation may be related to the recently proposed "sodium-potassium switch".

引用

页码：543 / 546

页数：4

共 32 条

[1] STRUCTURES FOR POLYINOSINIC ACID AND POLYGUANYLIC ACID [J].