A COMPARISON OF 2 DIFFERENT PROCEDURES FOR THE EXTRACTION OF ORGANIC MUTAGENS FROM SEWAGE-SLUDGE

被引:23
作者
BROWN, KW
CHISUM, CP
THOMAS, JC
DONNELLY, KC
机构
[1] Soil and Crop Sciences Department Texas A, M University, College Station
关键词
D O I
10.1016/0045-6535(90)90082-5
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The efficiency of the standard Soxhlet extraction procedure versus three different protocols for the Tecator Automatic Soxhlet extractor were compared for the extraction of organic mutagens from municipal sewage sludge using methanol and methylene chloride as solvents. The weights of the organic residues recovered from the standard Soxhlet and all Tecator procedures were very similar. Both the Soxhlet and Tecator 5 55 (boiling time/rinsing time) procedures yielded approximately the same level of mutagenic activity in the sewage sludge. However, the organic residue extracted from the sludge using either the Tecator 5 115, or the Tecator 10 50 failed to induce positive mutagenic responses in the bioassay. A comparison of the mutagenic potential of the residue obtained from triplicate extractions using both the Tecator 5 55 and the standard Soxhlet extraction indicated that both procedures yielded a reproducible mutagenic response within the variability of the bioassay. The data indicate that the Tecator 5 55 extraction, which is more rapid and requires less solvent, provides adequate extraction of organic mutagens from municipal sewage sludge. © 1990.
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页码:13 / 20
页数:8
相关论文
共 14 条
  • [1] METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST
    AMES, BN
    MCCANN, J
    YAMASAKI, E
    [J]. MUTATION RESEARCH, 1975, 31 (06): : 347 - 363
  • [2] Bridle T. R., 1983, Environmental effects of organic and inorganic contaminants in sewage sludge, P27
  • [3] BROWN KW, 1988, STABILITY MOBILITY M, P1
  • [4] EVALUATING STATISTICAL-ANALYSES AND REPRODUCIBILITY OF MICROBIAL MUTAGENICITY ASSAYS
    CHU, KC
    PATEL, KM
    LIN, AH
    TARONE, RE
    LINHART, MS
    DUNKEL, VC
    [J]. MUTATION RESEARCH, 1981, 85 (03): : 119 - 132
  • [5] DONNELLY KC, 1988, ASTM STP, V976, P288
  • [6] HOKPE PK, 1984, ENVIRON SCI TECHNOL, V18, P909
  • [7] HOKPE PK, 1982, ENVIRON SCI TECHNOL, V16, P140
  • [8] JACOBS LW, 1978, ENVIRON HEALTH PERSP, V23, P1
  • [9] JOHNSON BD, 1986, OCT EPA S APPL SHORT
  • [10] JOHNSTON JB, 1983, FW38 U ILL I ENV STU