QUANTITATION OF MOLYBDOPTERIN OXIDATION-PRODUCT IN WILD-TYPE AND MOLYBDENUM COFACTOR DEFICIENT MUTANTS OF CHLAMYDOMONAS-REINHARDTII

被引：8

作者：

AGUILAR, M ^{[1
]}

CARDENAS, J ^{[1
]}

FERNANDEZ, E ^{[1
]}

机构：

[1] UNIV CORDOBA,FAC CIENCIAS,DEPT BIOQUIM BIOL MOLEC & FISIOL,E-14071 CORDOBA,SPAIN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1160卷 / 03期

关键词：

MOLYBDENUM COFACTOR; MOLYBDOPTERIN; MOLYBDOENZYME; (CHLAMYDOMONAS-REINHARDTII);

D O I：

10.1016/0167-4838(92)90087-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A simple and reliable procedure of oxidation of molybdenum cofactor (MoCo) from molybdoenzymes by autoclaving samples at 120-degrees-C for 20 min yielded a single predominant fluorescent species that could be quantitatively determined by reverse phase high performance liquid chromatography. This method allowed detection and quantitation of molybdopterin in cell-free extracts of the green alga Chlamydomonas reinhardtii. The MoCo oxidation product from C reinhardtii has the same chromatographic and spectral properties as that of milk xanthine oxidase and chicken liver sulfite oxidase. The oxidized species was also detected in molybdenum cofactor mutants of Chlamydomonas reinhardtii defective at the nit-3, nit-4, nit-5/nit-6 and nit-7 loci, which strongly suggests that active molybdenum cofactor itself is not directly involved in the control of its own biosynthetic pathway.

引用

页码：269 / 274

页数：6

共 18 条

[1]

REGULATION OF MOLYBDENUM COFACTOR SPECIES IN THE GREEN-ALGA CHLAMYDOMONAS-REINHARDTII [J].