Improved method for pro-urokinase refolding with inclusion body from recombinant Escherichia coli

被引：1

作者：

Kubo, M ^{[1
]}

Nishi, A ^{[1
]}

机构：

[1] TOSOH CORP,SCI INSTRUMENT DEV DEP,AYASE,KANAGAWA 252,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1995年 / 80卷 / 06期

关键词：

protein folding; pro-urokinase; inclusion body; recombinant protein;

D O I：

10.1016/0922-338X(96)87745-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We developed an efficient inclusion body pro-urokinase refolding method from recombinant Escherichia coli. The protein was efficiently refolded when a heat treatment was applied to a protein denaturing solution containing guanidine hydrochloride. The total enzyme activity and the specific activity in response to the 50 degrees C heat treatment compared to normal method (25 degrees C) were enhanced about 10 and 25%, respectively. Moreover, enhanced protein refolding was also observed in the case of a reduced protein concentration in the protein refolding solution. The result indicates that correct protein folding is closely related to the protein concentration in the refolding solution.

引用

页码：622 / 624

页数：3

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