COLOR SELECTION WITH A HYGROMYCIN-RESISTANCE-BASED ESCHERICHIA COLI-MYCOBACTERIAL SHUTTLE VECTOR

被引：61

作者：

HOWARD, NS

GOMEZ, JE

KO, C

BISHAI, WR

机构：

[1] JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT MOLEC MICROBIOL & IMMUNOL,BALTIMORE,MD 21205

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT MED,DIV INFECT DIS,BALTIMORE,MD 21205

来源：

GENE | 1995年 / 166卷 / 01期

关键词：

SHUTTLE PLASMID; MYCOBACTERIA; MULTIPLE CLONING SITE; COLOR SELECTION; HYGROMYCIN GENE; LACZ GENE;

D O I：

10.1016/0378-1119(95)00597-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Hygromycin-resistance (Hy(R))-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZ alpha (encoding the alpha-polypeptide fragment of beta-galactosidase (beta Gal))-multiple cloning site cassette into a Hy(R)-based shuttle vector to generate a plasmid with nine unique cloning sites and the added feature of beta Gal color selection in appropriate E. coli host strains.

引用

页码：181 / 182

页数：2

共 8 条

[1] BIOLUMINESCENCE METHOD TO EVALUATE ANTIMICROBIAL AGENTS AGAINST MYCOBACTERIUM-AVIUM
COOKSEY, RC
MORLOCK, GP
BEGGS, M
CRAWFORD, JT
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (03) : 754 - 756
[2] SUPERINFECTION IMMUNITY OF MYCOBACTERIOPHAGE-L5 - APPLICATIONS FOR GENETIC-TRANSFORMATION OF MYCOBACTERIA
DONNELLYWU, MK
JACOBS, WR
HATFULL, GF
[J]. MOLECULAR MICROBIOLOGY, 1993, 7 (03) : 407 - 417
[3] TRANSFORMATION OF MYCOBACTERIAL SPECIES USING HYGROMYCIN RESISTANCE AS SELECTABLE MARKER
GARBE, TR
BARATHI, J
BARNINI, S
ZHANG, Y
ABOUZEID, C
TANG, D
MUKHERJEE, R
YOUNG, DB
[J]. MICROBIOLOGY-SGM, 1994, 140 : 133 - 138
[4] Hatfull G F, 1993, Trends Microbiol, V1, P310, DOI 10.1016/0966-842X(93)90008-F
[5] COMPLETE NUCLEOTIDE-SEQUENCE OF PAL5000, A PLASMID FROM MYCOBACTERIUM-FORTUITUM
RAUZIER, J
MONIZPEREIRA, J
GICQUELSANZEY, B
[J]. GENE, 1988, 71 (02) : 315 - 321
[6] LYSOGENY AND TRANSFORMATION IN MYCOBACTERIA - STABLE EXPRESSION OF FOREIGN GENES
SNAPPER, SB
LUGOSI, L
JEKKEL, A
MELTON, RE
KIESER, T
BLOOM, BR
JACOBS, WR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) : 6987 - 6991
[7] GENETIC-ANALYSIS OF SUPEROXIDE-DISMUTASE, THE 23 KILODALTON ANTIGEN OF MYCOBACTERIUM-TUBERCULOSIS
ZHANG, Y
LATHIGRA, R
GARBE, T
CATTY, D
YOUNG, D
[J]. MOLECULAR MICROBIOLOGY, 1991, 5 (02) : 381 - 391
[8] THE CATALASE PEROXIDASE GENE AND ISONIAZID RESISTANCE OF MYCOBACTERIUM-TUBERCULOSIS
ZHANG, Y
HEYM, B
ALLEN, B
YOUNG, D
COLE, S
[J]. NATURE, 1992, 358 (6387) : 591 - 593

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