CALCIUM STOICHIOMETRY DETERMINATION FOR CALCIUM-BINDING PROTEINS BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY

被引：113

作者：

HU, PF ^{[1
]}

YE, QZ ^{[1
]}

LOO, JA ^{[1
]}

机构：

[1] PARKE DAVIS PHARMACEUT RES, ANN ARBOR, MI 48105 USA

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 23期

关键词：

D O I：

10.1021/ac00095a013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Electrospray ionization mass spectrometry was used for the determination of calcium-binding stoichiometry for calcium-binding proteins. Bovine calmodulin, bovine alpha-lactalbumin, and rabbit parvalbumin were found to bind specifically to 4, 1, and 2 Ca2+ ions, respectively, in agreement with previously reported results obtained by using other physical methods. This mass spectrometry method could also be applied to proteins that bind more than one type of metal ion. The Zn2+- and Ca2+-binding stoichiometries for human stromelysin catalytic domain were determined to be 3 and 2, respectively.

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页码：4190 / 4194

页数：5

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