METHIONINE-INDEPENDENCE, TUMORIGENICITY AND ONCOGENE EXPRESSION OF RAT HEPATOCARCINOMA CELLS

被引：10

作者：

CASSINGENA, R ^{[1
]}

LAFARGEFRAYSSINET, C ^{[1
]}

PAINCHAULT, V ^{[1
]}

ESTRADE, S ^{[1
]}

NARDEUX, P ^{[1
]}

FRAYSSINET, C ^{[1
]}

机构：

[1] INST RECH SCI CANC, PATHOL CELLULAIRE LAB, PLURIDISCIPLINAIRE RECH CANC LAB, F-94801 VILLEJUIF, FRANCE

来源：

BIOLOGY OF THE CELL | 1990年 / 69卷 / 02期

关键词：

hepatocarcinoma; methionine deficiency; oncogenes; tumorigenicity;

D O I：

10.1016/0248-4900(90)90337-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Methionine-dependent (Met-Hcy+) revertant sublines were isolated from a methionine-independent (Met+Hcy-) rat hepatocarcinoma cell line (LF). Characterization of these sublines has shown that long-term culture in methionine-deprived medium significantly decreases their ability to grow in vitro, their tumorigenicity in new-born syngeneic animals and their level of transcription of 3 oncogenes (c-Ki-ras, c-Ha-ras, c-myc) involved in hepatic growth. These data strongly support a relationship between methioninedependence and ability to grow in vivo as tumors. A shift in methionine-supplemented medium for several cell generations of the various sublines grown in methionine deprived medium, has revealed that under these conditions, the cells maintain a diminished in vitro growth and tumorigenic capacity, despite an increased transcription of c-Ki-ras, c-Ha-ras and c-myc. This points out the complexity of the relationship between the level of activity of some oncogenes and the expression of the malignant phenotypes. © 1990.

引用

页码：113 / 118

页数：6

共 14 条

[1] C-H-RAS AND C-K-RAS GENE HYPOMETHYLATION IN THE LIVERS AND HEPATOMAS OF RATS FED METHYL-DEFICIENT, AMINO ACID-DEFINED DIETS [J].

BHAVE, MR ;

WILSON, MJ ;

POIRIER, LA .

CARCINOGENESIS, 1988, 9 (03) :343-348

[2] ASSOCIATION OF SIBA TREATMENT AND A MET-DEPLETED DIET INHIBITS INVITRO GROWTH AND INVIVO METASTATIC SPREAD OF EXPERIMENTAL TUMOR-CELL LINES [J].

BREILLOUT, F ;

POUPON, MF ;

BLANCHARD, P ;

LASCAUX, V ;

ECHINARDGARIN, P ;

ROBERTGERO, M .

CLINICAL & EXPERIMENTAL METASTASIS, 1988, 6 (01) :3-16

[3] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[4] ALTERED METHIONINE METABOLISM, DNA METHYLATION AND ONCOGENE EXPRESSION IN CARCINOGENESIS - A REVIEW AND SYNTHESIS [J].

HOFFMAN, RM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 738 (1-2) :49-87

[5]

JUDDE JG, 1989, CANCER RES, V49, P4859

[6] STRUCTURAL CORRELATES OF CELLULAR TUMORIGENICITY AND ANCHORAGE INDEPENDENCE IN TRANSFORMED FIBROBLASTS [J].

KAHN, P ;

HELLER, D ;

SHIN, S .

CYTOGENETICS AND CELL GENETICS, 1983, 36 (04) :605-611

[7]

LAFARGEFRAYSSIN.C, 1988, LIVER CELLS DRUGS, V164, P465

[8] AGAR SUSPENSION CULTURE FOR SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS [J].

MACPHERSON, I ;

MONTAGNIER, L .

VIROLOGY, 1964, 23 (02) :291-&

[9] NUCLEOTIDE-SEQUENCE OF RIGHTWARD OPERATOR OF PHAGE LAMBDA [J].

MANIATIS, T ;

JEFFREY, A ;

KLEID, DG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (03) :1184-1188

[10]

MANIATIS T, 1982, MOL CLONING LABORATO, P187

← 1 2 →