MONITORING OF PROTEIN PRODUCT FORMATION DURING FERMENTATION WITH FAST PROTEIN LIQUID-CHROMATOGRAPHY

被引：9

作者：

GUSTAFSSON, JG

FREJ, AK

HEDMAN, P

机构：

[1] Pharmacia AB, Uppsala, Swed, Pharmacia AB, Uppsala, Swed

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1986年 / 28卷 / 01期

关键词：

CHEMICAL REACTIONS - Fermentation - CHROMATOGRAPHIC ANALYSIS - Liquid - ENZYMES - ION EXCHANGERS;

D O I：

10.1002/bit.260280104

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method is described using fast protein liquid chromatography (FPLC) for the monitoring of protein formation during fermentation. The procedure consists of centrifugation to recover the cells, sonication of the cells, centrifugation to remove cell debris, and analysis of supernatant on a column of Mono Q (a strong anion exchanger). Analysis of peak areas provides quantitative determinations of product concentration. Maintenance and life of the Mono Q column is discussed. We find that FPLC is a convenient method for measuring products in cell homogenates because it gives rapid, highly resolved separations.

引用

页码：16 / 20

页数：5

共 4 条

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HAFF, LA ;

FAGERSTAM, LG ;

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[3] COLUMN LIFETIME IN FAST PROTEIN LIQUID-CHROMATOGRAPHY [J].

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[4]

SODERBERG L, 1983, PROTIDES BIOL FLUIDS, V30, P635

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