A PHYSIOLOGICAL-ROLE FOR DNA SUPERCOILING IN THE ANAEROBIC REGULATION OF COLICIN GENE-EXPRESSION

被引：10

作者：

MALKHOSYAN, SR ^{[1
]}

PANCHENKO, YA ^{[1
]}

REKESH, AN ^{[1
]}

机构：

[1] ACAD SCI USSR,INST MOLEC GENET,46 KURCHATOV SQ,MOSCOW 123182,USSR

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 225卷 / 02期

关键词：

ANAEROBIOSIS; DNA SUPERCOILING; GENE EXPRESSION; COLICINS;

D O I：

10.1007/BF00269868

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanism of anaerobic regulation of synthesis of colicins E1, E2, E3, K and D was studied. It was found that anaerobiosis significantly increases expression of the genes for colicins E1, E2, E3, K, and D. Experiments with novobiocin (a DNA gyrase inhibitor) showed that colicin synthesis in minicells and derepressed colicin synthesis in cells are dramatically reduced by relaxation of DNA supercoiling. A good correlation was observed between the levels of colicin synthesis and plasmid DNA supercoiling and the degree of aeration of the cultures. Thus, the regulation of colicin gene expression in response to a change in aeration appears to be mediated by environmentally induced variations in DNA supercoiling.

引用

页码：342 / 345

页数：4

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