A MATHEMATICAL-MODEL OF HOMOLOGOUS RECOMBINATION IN CULTURED-CELLS

被引：4

作者：

BASTIN, M

STONGE, L

BOUCHARD, L

机构：

[1] Department of Microbiology, University of Sherbrooke, Sherbrooke

来源：

JOURNAL OF THEORETICAL BIOLOGY | 1992年 / 156卷 / 04期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/S0022-5193(05)80641-1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This work presents a model describing the rate of recombination between homologous segments of DNA stably integrated into the genome of cultured cells. The model has been applied to rat cell lines carrying the polyomavirus middle T oncogene and a functional origin of viral DNA replication. Introduction of the gene coding for the polyoma large T antigen or the SV40 large T antigen into cells by DNA transfection promotes homologous recombination in the resident viral inserts with rates varying between 0·1 × 10-3 and 3·7 × 10-1 per cell generation. © 1992 Academic Press Limited.

引用

收藏

页码：513 / 524

页数：12

相关论文

共 18 条

[1] SEQUENCES FROM POLYOMAVIRUS AND SIMIAN VIRUS-40 LARGE T-GENES CAPABLE OF IMMORTALIZING PRIMARY RAT EMBRYO FIBROBLASTS [J].

ASSELIN, C ;

BASTIN, M .

JOURNAL OF VIROLOGY, 1985, 56 (03) :958-968

[2] MUTATION IN THE POLYOMAVIRUS GENOME THAT ACTIVATES THE PROPERTIES OF LARGE-T ASSOCIATED WITH NEOPLASTIC TRANSFORMATION [J].

ASSELIN, C ;

VASSMARENGO, J ;

BASTIN, M .

JOURNAL OF VIROLOGY, 1986, 57 (01) :165-172

[3] GENE CONVERSION - SOME IMPLICATIONS FOR IMMUNOGLOBULIN GENES [J].

BALTIMORE, D .

CELL, 1981, 24 (03) :592-594

[4] PATTERNS OF INTEGRATION OF DNA MICRO-INJECTED INTO CULTURED MAMMALIAN-CELLS - EVIDENCE FOR HOMOLOGOUS RECOMBINATION BETWEEN INJECTED PLASMID DNA-MOLECULES [J].

FOLGER, KR ;

WONG, EA ;

WAHL, G ;

CAPECCHI, MR .

MOLECULAR AND CELLULAR BIOLOGY, 1982, 2 (11) :1372-1387

[5] MODEL FOR HOMOLOGOUS RECOMBINATION DURING TRANSFER OF DNA INTO MOUSE L-CELLS - ROLE FOR DNA ENDS IN THE RECOMBINATION PROCESS [J].

LIN, FL ;

SPERLE, K ;

STERNBERG, N .

MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (06) :1020-1034

[6] EVIDENCE FOR INTRACHROMOSOMAL GENE CONVERSION IN CULTURED MOUSE CELLS [J].

LISKAY, RM ;

STACHELEK, JL .

CELL, 1983, 35 (01) :157-165

[7] HOMOLOGOUS RECOMBINATION BETWEEN REPEATED CHROMOSOMAL SEQUENCES IN MOUSE CELLS [J].

LISKAY, RM ;

STACHELEK, JL ;

LETSOU, A .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1984, 49 :183-189

[8]

Luria SE, 1943, GENETICS, V28, P491

[9] DIVERSITY ACHIEVED BY DIVERSE MECHANISMS - GENE CONVERSION IN DEVELOPING B-CELLS OF THE CHICKEN [J].

MAIZELS, N .

CELL, 1987, 48 (03) :359-360

[10] A HYPERCONVERSION MECHANISM GENERATES THE CHICKEN LIGHT CHAIN PREIMMUNE REPERTOIRE [J].

REYNAUD, CA ;

ANQUEZ, V ;

GRIMAL, H ;

WEILL, JC .

CELL, 1987, 48 (03) :379-388