A SIMPLE ENHANCEMENT METHOD FOR THE SILVER GOLD-INTENSIFIED DIAMINOBENZIDINE REACTION IN THE LIGHT MICROSCOPIC IMMUNOPEROXIDASE TECHNIQUE

被引：15

作者：

GOTO, S ^{[1
]}

NAGAHIRO, S ^{[1
]}

USHIO, Y ^{[1
]}

HOFER, W ^{[1
]}

机构：

[1] MAX PLANCK INST BRAIN RES,ELECTRON MICROSCOPY LAB,W-6000 FRANKFURT,GERMANY

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1992年 / 40卷 / 09期

关键词：

DIAMINOBENZIDINE; SILVER GOLD INTENSIFICATION; IMMUNOCYTOCHEMISTRY; URANYL NITRATE ENHANCEMENT;

D O I：

10.1177/40.9.1506678

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We describe a simple and sensitive method for enhancement of the silver-gold-intensified 3,3'-diaminobenzidine (DAB) reaction demonstrating peroxidase activity. After completing silver-gold intensification of the preparations immunostained by the avidin-biotin peroxidase method with DAB as the chromogen, the preparations were immersed in a solution containing uranyl nitrate. This new method appeared to increase the sensitivity by at least one order of magnitude as compared with silver-gold intensification alone.

引用

页码：1423 / 1425

页数：3

共 7 条

[1]

EER JM, 1942, REZEPTE FOTOGRAFIE

[2] HIGH-GRADE INTENSIFICATION OF THE END-PRODUCT OF THE DIAMINOBENZIDINE REACTION FOR PEROXIDASE HISTOCHEMISTRY [J].

GALLYAS, F ;

GORCS, T ;

MERCHENTHALER, I .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1982, 30 (02) :183-184

[3] SUBDIVISIONAL INVOLVEMENT OF NIGROSTRIATAL LOOP IN IDIOPATHIC PARKINSONS-DISEASE AND STRIATONIGRAL DEGENERATION [J].

GOTO, S ;

HIRANO, A ;

MATSUMOTO, S .

ANNALS OF NEUROLOGY, 1989, 26 (06) :766-770

[4] USE OF AVIDIN-BIOTIN-PEROXIDASE COMPLEX (ABC) IN IMMUNOPEROXIDASE TECHNIQUES - A COMPARISON BETWEEN ABC AND UNLABELED ANTIBODY (PAP) PROCEDURES [J].

HSU, SM ;

RAINE, L ;

FANGER, H .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1981, 29 (04) :577-580

[5] APPLICATION OF THE SILVER GOLD INTENSIFIED 3,3'-DIAMINOBENZIDINE CHROMOGEN TO THE LIGHT AND ELECTRON-MICROSCOPIC DETECTION OF THE LUTEINIZING-HORMONE-RELEASING HORMONE SYSTEM OF THE RAT-BRAIN [J].

LIPOSITS, Z ;

SETALO, G ;

FLERKO, B .

NEUROSCIENCE, 1984, 13 (02) :513-525

[6] REINNERVATION OF CEREBELLAR PURKINJE-CELLS BY CLIMBING FIBERS SURVIVING A SUBTOTAL LESION OF THE INFERIOR OLIVE IN THE ADULT-RAT .2. SYNAPTIC ORGANIZATION ON REINNERVATED PURKINJE-CELLS [J].

ROSSI, F ;

VANDERWANT, JJL ;

WIKLUND, L ;

STRATA, P .

JOURNAL OF COMPARATIVE NEUROLOGY, 1991, 308 (04) :536-554

[7] SYNAPTIC RELATIONSHIPS BETWEEN NEURONS CONTAINING VASOPRESSIN, GASTRIN-RELEASING PEPTIDE, VASOACTIVE INTESTINAL POLYPEPTIDE, AND GLUTAMATE-DECARBOXYLASE IMMUNOREACTIVITY IN THE SUPRACHIASMATIC NUCLEUS - DUAL ULTRASTRUCTURAL IMMUNOCYTOCHEMISTRY WITH GOLD-SUBSTITUTED SILVER PEROXIDASE [J].

VANDENPOL, AN ;

GORCS, T .

JOURNAL OF COMPARATIVE NEUROLOGY, 1986, 252 (04) :507-521

← 1 →