HIGH-FREQUENCY TRANSFORMATION FROM CULTURED COTYLEDONS OF ARABIDOPSIS-THALIANA ECOTYPES C24 AND LANDSBERG ERECTA

被引：6

作者：

BARGHCHI, M ^{[1
]}

TURGUT, K ^{[1
]}

SCOTT, R ^{[1
]}

DRAPER, J ^{[1
]}

机构：

[1] UNIV LEICESTER,DEPT BOT,LEICESTER LE1 7RH,W YORKSHIRE,ENGLAND

来源：

PLANT GROWTH REGULATION | 1994年 / 14卷 / 01期

关键词：

ARABIDOPSIS; REGENERATION; COTYLEDON; TRANSGENIC PLANTS; ANTIBIOTICS;

D O I：

10.1007/BF00024142

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Procedures were developed for rapid and prolific adventitious shoot regeneration of Arabidopsis thaliana (L.) Heynh ecotypes ''Landsberg erect'' and ''C24'' from cotyledon explants at 90-100% efficiency. Immature cotyledons had the highest shoot regeneration efficiency. Prolific regeneration was achieved in Murashige and Skoog medium (MS) supplemented with 0.1-0.4 mg(-1) naphthalene acetic acid (NAA) and 1.0 mg(-1) 6-benzylaminopurine (BAP) within 2 weeks. The regenerated plants had a normal phenotype and produced fertile flowers and set seeds. The above regeneration protocol was used to develop a transformation method using disarmed strains of Agrobacterium tumefaciens strains pGV3850::pH1121 based on kanamycin (Km) selection. Transgenic shoots were produced within 2-3 weeks after inoculation. Transformation of shoots was confirmed by GUS histochemical assay, as well as southern blot hybridization.

引用

页码：61 / 67

页数：7

共 23 条

[1]

ACEDO GN, 1986, PLANT CELL TISS ORG, V6, P109

[2]

AN G, 1986, PLANT PHYSIOL, V81, P31

[3]

BARGHCHI M, 1989, BIOTECHNOLOGY AGR FO

[4] EFFICIENT TRANSFORMATION OF ARABIDOPSIS-THALIANA USING DIRECT GENE-TRANSFER TO PROTOPLASTS [J].

DAMM, B ;

SCHMIDT, R ;

WILLMITZER, L .

MOLECULAR AND GENERAL GENETICS, 1989, 217 (01) :6-12

[5]

DRAPER J, 1988, PLANT GENETIC TRANSF

[6] THE PRODUCTION OF CALLUS CAPABLE OF PLANT-REGENERATION FROM IMMATURE EMBRYOS OF NUMEROUS ZEA-MAYS GENOTYPES [J].

DUNCAN, DR ;

WILLIAMS, ME ;

ZEHR, BE ;

WIDHOLM, JM .

PLANTA, 1985, 165 (03) :322-332

[7] RAPID AND EFFICIENT REGENERATION OF PLANTS FROM EXPLANTS OF ARABIDOPSIS-THALIANA [J].

FELDMANN, KA ;

MARKS, MD .

PLANT SCIENCE, 1986, 47 (01) :63-69

[8] AGROBACTERIUM-MEDIATED TRANSFORMATION OF GERMINATING-SEEDS OF ARABIDOPSIS-THALIANA - A NON-TISSUE CULTURE APPROACH [J].

FELDMANN, KA ;

MARKS, MD .

MOLECULAR AND GENERAL GENETICS, 1987, 208 (1-2) :1-9

[9] PLANT-REGENERATION FROM CELL-SUSPENSION CULTURES OF ARABIDOPSIS-THALIANA HEYNH [J].

GLEDDIE, S .

PLANT CELL REPORTS, 1989, 8 (01) :1-5

[10]

GOTO N, 1979, HEYNH, V54, P303

← 1 2 3 →