CLONING AND SEQUENCE-ANALYSIS OF THE GENE ENCODING THE DNA POLYMERASE-I FROM MYCOBACTERIUM-TUBERCULOSIS

被引：17

作者：

HUBERTS, P

MIZRAHI, V

机构：

[1] S AFRICAN INST MED RES,MOLEC BIOL UNIT,JOHANNESBURG 2000,SOUTH AFRICA

[2] UNIV WITWATERSRAND,SCH MED,DEPT HEMATOL,JOHANNESBURG 2000,SOUTH AFRICA

来源：

GENE | 1995年 / 164卷 / 01期

关键词：

DNA REPAIR; DNA REPLICATION; MYCOBACTERIUM LEPRAE; POLL;

D O I：

10.1016/0378-1119(95)00453-D

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The polA gene (encoding DNA polymerase I) from Mycobacterium tuberculosis was cloned using an internal gene segment probe generated by PCR amplification of genomic DNA [Mizrahi et al., Gene 136 (1993) 287-290]. The gene encodes a polypeptide 904 amino acids (aa) in length that shares 89% identity with a 911-aa homologue from Mycobacterium leprae. The polypeptide has all of the primary structural elements necessary for DNA polymerase and 5'-3' exonuclease activity, but lacks the motifs required for an associated 3'-5' exonuclease (proofreading) activity.

引用

页码：133 / 136

页数：4

共 21 条

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