CHLOROPHYLL FLUORESCENCE ANALYSIS AND IMAGING IN PLANT STRESS AND DISEASE

被引：44

作者：

DALEY, PF

机构：

[1] Environmental Restoration Division, Lawrence Livermore National Laboratory, Livermore, CA, 94550

来源：

CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE | 1995年 / 17卷 / 02期

关键词：

D O I：

10.1080/07060669509500708

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Quantitative analysis of chlorophyll fluorescence transients and quenching has evolved rapidly in the last decade. Instrumentation capable of fluorescence detection in bright actinic light has been used in conjunction with gas exchange analysis to build an empirical foundation relating quenching parameters to photosynthetic electron transport, the state of the photoapparatus, and carbon fixation. We have developed several instruments that collect video images of chlorophyll fluorescence. Digitized versions of these images can be manipulated as numerical data arrays, supporting generation of quenching maps that represent the spatial distribution of photosynthetic activity in leaves. We have applied this technology to analysis of fluorescence quenching during application of stress hormones, herbicides, and physical stresses, including drought and sudden changes in humidity of the atmosphere surrounding leaves, and during stomatal oscillations in high CO2 We describe a recently completed portable fluorescence imaging system utilizing LED illumination and a consumer-grade camcorder, that will be used in long-term, nondestructive field studies of plant virus infections.

引用

页码：167 / 173

页数：7

共 32 条

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