RESPONSES OF MOUSE FAST AND SLOW SKELETAL-MUSCLE TO STREPTOZOTOCIN DIABETES - MYOSIN ISOENZYMES AND PHOSPHORUS METABOLITES

A condition similar to insulin-dependent diabetes mellitus (IDDM) was induced in male CD-I mice by injection of streptozotocin (STZ). Five weeks after treatment, the fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus (SOL) muscles were isolated for analysis. Phosphorous metabolites were quantified by P-31-NMR and HPLC, native myosin was characterized electrophoretically, and activities of metabolic enzymes were measured spectrophotometrically. Relative to control animals, STZ-diabetes resulted in a significant 32% decrease in the FM, isoform of myosin in EDL and a 24% decrease in IM myosin of SOL. Mass-specific activities of phosphofructokinase, citrate synthase, and cytochrome oxidase were significantly lower in SOL from STZ-diabetic mice than in controls by 23, 18, and 36%, respectively. Intracellular ATP was significantly lower in SOL from STZ-diabetic mice than in controls (3.44 +/- 0.20 mu mol g(-1) wet weight vs. 4.61 +/- 0.20 mu mol g(-1), respectively), as was creatine phosphate (11.98 +/- 0.80 mu mol g(-1) wet weight vs. 14.22 +/- 0.44 mu mol g(-1)). In contrast to results from SOL, there were no significant changes in phosphorus metabolites or enzyme activity in EDL. These results show that the effects of IDDM on levels of phosphorus containing metabolites and maximal activities of key regulatory enzymes in muscle are markedly fiber-type specific. It is suggested that the muscle type-specific effects of STZ-diabetes may be a consequence of differential accumulation of intracellular fatty acids.