MODELING THE GROWTH OF A BATCH CULTURE OF PLANT-CELLS - A CORPUSCULAR APPROACH

被引：18

作者：

DEGUNST, MCM

HARKES, PAA

VAL, J

VANZWET, WR

LIBBENGA, KR

机构：

[1] LEIDEN STATE UNIV,DEPT PLANT MOLEC BIOL,2312 AV LEIDEN,NETHERLANDS

[2] LEIDEN STATE UNIV,DEPT MATH,2312 AV LEIDEN,NETHERLANDS

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 01期

关键词：

batch culture; cell division; corpuscular model; differentiation; Growth model; plant cell;

D O I：

10.1016/0141-0229(90)90182-P

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A stochastic model is formulated for the cell cycle of plant cells during batch culture. In the model two types of cells are distinguished: dividing cells and nondividing, differentiating cells. The cell cycle duration is assumed to depend on the substrate concentration in the medium, whereas the number of newly born cells, which will again divide, is supposed to depend on the hormone concentration in the medium. The consequences of the model for the growth of the total plant cell culture in terms of cell numbers are considered. Experiments performed in order to test the model are described and the results are discussed. This approach of describing plant cell population growth by means of a corpuscular mathematical model is a first attempt to obtain a better understanding of the regulation of division, differentiation, growth, and product formation of plant cells grown under different conditions. © 1990.

引用

页码：61 / 71

页数：11

共 19 条

[1]

Alberts B., 1983, MOL BIOL CELL

[2]

BAYLISS MW, 1985, CELL DIVISION CYCLE

[3]

BROWN JT, 1986, SECONDARY METABOLISM

[4]

COLLINGE MA, 1986, SECONDAARY METABOLIS